Niki PCR itaziguye

PCR itaziguye nuburyo bwo kongera ADN biturutse kumaraso, inyama zinyamanswa, umurizo wimbeba, selile, ugutwi kwimbeba, amafi ya zebra, amagi y amafi, amababi y ibimera, imbuto cyangwa izindi ngero zumwimerere zidafite umubiri udakoze intambwe yo kwigunga no kweza ADN.Tekinike ya PCR itaziguye irashobora kugabanya cyane igihe cyubushakashatsi, nigiciro muri genotyping hamwe nimishinga myinshi.Itanga kandi amahitamo meza mugihe uhuye ningorane zo kongera umubare muto wicyitegererezo aho intambwe yo kweza ishobora gutera igihombo cyicyitegererezo.

PCR ya PCR ya FOREGENE ifite imbaraga zo guhangana n’ingutu, kubera imbaraga za FOREGENE zatanzwe na Taq enzyme (ipatanti yemewe: ZL20161034512.0 (Ubushinwa), EP3450560 (Uburayi), PCT / CN2017 / 082154 (Amerika), ipatanti 6894926 (Ubuyapani)).Enzyme ya Taq ikoresha ikorana buhanga rya ADN kugirango yongere guhuza ADN ihuza akarere ka acide nucleic aside ihuza poroteyine hamwe na polymerase ya Taq irwanya ubushyuhe kugirango yubake ADN yerekana urugero rwinshi.Gutezimbere gushyushye-gutangira Taq igumana imirimo itandukanye ya polymerase yumwimerere ya ADN.Iyi misemburo ya Taq irashobora guhuza na ADN yerekana icyitegererezo hanyuma igatangiza synthesis ya ADN mubishusho bito hamwe nibidukikije bigoye.

Kurugero, biroroshye nko kongeramo selile, igihingwa (amababi akiri mato, umuzi cyangwa imbuto) cyangwa icyitegererezo cyinyamanswa kuri buffer, no kongeramo master idasanzwe ivanze na primers yihariye mumiyoboro ya PCR hanyuma ukayinyuza mumagare yumuriro.Ibikoresho byiza nibyiza kubisesengura binini byerekana urugero aho kubika umwanya ari ngombwa cyane.

Sisitemu ya reaction ya PCR irimo enzyme ya UNG na dUTP yatoranijwe ukurikije ibisabwa mubushakashatsi, ishobora kwirinda neza umwanda uterwa nibicuruzwa byongera PCR.