Umurizo wimbeba PCR Kit Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)
Ibisobanuro:
Koresha mu buryo butaziguye imbeba umurizo lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
Ibisobanuro
Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer kugirango irekure vuba ADN ya genomic kuva umurizo wimbeba, ugutwi, imitsi hamwe nizindi ngero za tissue kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.
Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.
2 × M1-PCR BiroroshyeTMKuvanga bifite kwihanganira bikomeye PCR yerekana inhibitor, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction ya reaction, optimizer ya PCR na stabilisateur.
D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.
Ibisobanuro
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ibigize ibikoresho
| Igice cya I. | Depite Buffer |
| Foregene Protease Yongeyeho | |
| 6 × ADN Yipakurura Buffer | |
| Igice cya II | 2 × M1 PCR BiroroshyeTMKuvanga |
| Amabwiriza | |
Ibiranga ibyiza
■Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
Icyitegererezo gikenewe ni gito, nkibice 5mg byimbeba cyangwa umurizo wimbeba 2-5mm.
■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
Ibipimo by'ibikoresho
Gushyira mu bikorwa: Imbeba umurizo, amatwi yimbeba, imitsi nizindi ngingo.
ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.
Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.
Urujya n'uruza rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Igice cya I cyiki gitabo kigomba kubikwa kuri 2-8℃.
Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8℃kubikwa igihe kirekire.
Reagent Foregene Protease ifite formula idasanzwe.Kugirango ibikorwa byayo bihamye, nyamuneka ubibike kuri 4℃amezi 12.
Reagent 6×ADN Yipakurura Buffer irashobora kubikwa kuri 4℃cyangwa -20℃igihe kirekire.
Igice cya II cyiki gikoresho kigomba kubikwa kuri -20℃.
Reagent 2×M1 PCR BiroroshyeTMKuvanga, niba bikoreshwa kenshi, birashobora kandi kubikwa kuri 4℃kubikwa igihe gito (koresha muminsi 10).
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