Ubushinwa Imbeba Umurizo PCR Kit-UNG Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping) Uwakoze nuwitanga |Foregene

Umurizo wimbeba PCR Kit-UNG Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)

Ibisobanuro:

Imbeba umurizo lysate ikoreshwa muburyo butaziguye nk'icyitegererezo cyo kongera PCR nta gukuramo ADN itandukanye, yihuta kandi yunvikana cyane.Wongeyeho UNG yo kurwanya umwanda kugirango ikureho ibyiza.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer kugirango irekure vuba ADN ya genomic kuva umurizo wimbeba, ugutwi, imitsi hamwe nizindi ngero za tissue kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × M1-PCR Biroroshye^TMKuvanga (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2 × M1-PCR Byoroshye^TMKuvanga (UNG), hanyuma ukongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo kikaba cyemeza umwihariko n'ukuri kwa amplification no gukumira ko hashobora kubaho ibizamini binini.Kwanduza ibicuruzwa bya PCR byabaye.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Depite Buffer
	Foregene Protease Yongeyeho
	6 × ADN Yipakurura Buffer
Igice cya II	2 × M1 PCR Biroroshye^TMKuvanga (UNG)
Amabwiriza

Ibiranga ibyiza

■Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

Icyitegererezo gikenewe ni gito, nkibice 5mg byimbeba cyangwa umurizo wimbeba 2-5mm.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

System Sisitemu yo kurwanya umwanda PCR 2×M1-PCR Biroroshye^TMKuvanga (UNG), bikuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.