-Ubushobozi buhagije bwo gukuraho gDNA, ishobora gukuraho gDNA mubishusho muminota 2.
-Uburyo bwiza bwo kwandukura sisitemu, bifata iminota 15 gusa kugirango urangize synthesis yumurongo wambere cDNA.
-Icyitegererezo cyoroshye: inyandikorugero zirimo ibintu byinshi bya GC hamwe nuburyo bugoye bwa kabiri birashobora kandi guhindurwa hamwe nubushobozi buhanitse.
-Ibihe byinshi-byunvikana sisitemu yo kwandukura, pg-urwego rwicyitegererezo nacyo gishobora kubona cDNA nziza.
-Uburyo bwo kwandukura sisitemu ifite ubushyuhe bwinshi, ubushyuhe bwiza ni 42℃, kandi iracyafite imikorere myiza yo kwandukura kuri 50℃.