Injangwe.No.DRT-01011/01012
Kuri selile itaziguye RT-qPCR ukoresheje selile 10-1,000,000,
no gukora RT-qPCR iturutse muri selile itabanje kweza RNA.
Ingirabuzimafatizo zirekuwe kugirango zirekure RNA kuri RT-qPCR;sisitemu yo kwihanganira cyane ituma bidakenewe kweza RNA no gukoresha mu buryo butaziguye lysates ya selile nka RNA yerekana ibisubizo bya RT.Byihuse kandi byoroshye;ibyiyumvo bihanitse, umwihariko ukomeye, hamwe no gutuza neza.
◮Byoroshye kandi byiza: hamwe na tekinoroji ya Cell Direct RT, ingero za RNA zirashobora kuboneka muminota 7 gusa.
◮Icyitegererezo gisabwa ni gito, nkuko selile 10 zishobora kugeragezwa.
Kwinjira cyane: irashobora gutahura vuba RNA mungirabuzimafatizo zifite 384, 96, 24, 12, 6-isahani nziza.
◮ADN Eraser irashobora gukuraho vuba genome zasohotse, bikagabanya cyane ingaruka kubisubizo byakurikiyeho.
◮Sisitemu nziza ya RT na qPCR ituma intambwe ebyiri RT-PCR ihinduranya inyandiko ikora neza kandi PCR ikarushaho kuba nziza, kandi ikarwanya RT-qPCR inhibitor.