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RT Byoroshyeᵀᴹ I Master Premix kumurongo wambere cDNA synthesis
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 25 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 ℃,kandi ifite imikorere myiza yo guhindura inyandiko.
- Ndetse na RNA yanduye (inzoga zigera kuri 45%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
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