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Igihe nyacyo PCR Byoroshyeᵀᴹ-SYBR Icyatsi I kit
Byoroshye-2X PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora
Byihariye-byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer
Ubukangurambaga bukabije - bushobora kumenya kopi ntoya yicyitegererezo
Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR
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RT-PCR Byoroshyeᵀᴹ I (Intambwe imwe)
◮Igikoresho kimwe cyintambwe ituma transcription transcription na PCR ikorerwa mumiyoboro imwe.Irakeneye gusa kongeramo inyandikorugero RNA, progaramu yihariye ya PCR na ddH yubusa ya RNase2O.
◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
◮Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
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RT-PCR Byoroshyeᵀᴹ II (Intambwe ebyiri)
◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
◮ Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
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Igihe nyacyo PCR Byoroshyeᵀᴹ-Taqman
◮Byoroshye - 2 × PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora
◮Byihariye-byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer
◮Ubukangurambaga bukabije - bushobora kumenya kopi ntoya yicyitegererezo
◮Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR
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Intwari ya PCR (Hamwe n'irangi)
◮Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;
◮Umuvuduko wihuse
◮Inyandikorugero nyinshi
◮Gukora neza cyane
◮Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;
◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.
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700bp Urwego rwa ADN
◮700bp ADN Urwego nigicuruzwa cyiteguye-gukoresha kandi kimaze kubamo 1x Loading Buffer.Urashobora gufata mu buryo butaziguye urugero rukwiye rwa Marker kuri electrophoreis ukurikije ubushakashatsi bukenewe.
700bpADN Marker Ⅰ igizwe na bande 7 za ADN, imirongo ya ADN ni: 100bp (50ng / 5μl), 200bp (30ng / 5μl), 300bp (30ng / 5μl), 400bp (40ng / 5μl), 500bp (25ng / 5μl)), 600bp (30ng / 5μl), 700bp (35ng / 5μl).
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2000bp Urwego rwa ADN
◮ 2000bp ADN Urwego ni igicuruzwa cyiteguye-gukoresha kandi kimaze kubamo 1x Loading Buffer.Urashobora gufata mu buryo butaziguye urugero rukwiye rwa Marker kuri electrophoreis ukurikije ubushakashatsi bukenewe.BM2000 ADN Marker igizwe na ADN 6, imirongo ya ADN ni: 100bp (50ng / 5μl), 250bp (50ng / 5μl), 500bp (50ng / 5μl), 750bp (75ng / 5μl), 1000bp (50ng / 5μl)), 2000bp (100ng / 5μl).
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5000bp Urwego rwa ADN
◮ 2000bp Urwego rwa ADNni biteguye-gukoresha-ibicuruzwa kandi bimaze kubamo 1x Loading Buffer.Urashobora gufata mu buryo butaziguye urugero rukwiye rwa Marker kuri electrophoreis ukurikije ubushakashatsi bukenewe.BM5000 ADN Marker igizwe na ADN 8, imirongo ya ADN ni: 100bp (50ng / 5μl), 250bp (50ng / 5μl), 500bp (50ng / 5μl), 750bp (75ng / 5μl), 1000bp (50ng / 5μl)), 2000bp (100ng / 5μl), 3000bp (30ng / 5μl)
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PCR Byoroshyeᵀᴹ (hamwe n'irangi)
2 × Intwari ya PCRTM Sisitemu yo kuvanga ifite kwihanganira cyane inhibitori za PCR kuruta sisitemu isanzwe ya PCR ivanze, kandi irashobora guhangana byoroshye na PCR ikwirakwizwa rya templates zitandukanye.Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq Intwari ituma reaction ya PCR igira ubushobozi bwo kongera amplification, umwihariko hamwe na sensitivite.
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PCR Byoroshyeᵀᴹ
Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq ADN Polymerase ituma PCR reaction ikora neza cyane, amplificateur na sensitivite.
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PCR Intwari
◮Ubudahemuka bwo hejuru: 6ibihe bya enzyme isanzwe ya Taq;
◮Byihuse aumuvuduko wa mplification:umuvuduko wa amplification ni 5-10sec / kb, bikubye inshuro 6-12 iy'umusemburo wa Taq usanzwe;
◮Inyandikorugero nyinshi: irashobora kwagura neza inyandikorugero zitandukanye za ADN zifite ibintu byinshi bya GC kandi bigoye kubyongera;
◮HejuruGukora neza: umubare wikizunguruka cyikigereranyo kiri munsi yumusemburo wa Taq usanzwe;
◮Stronger Kwihanganira ibidukikije: yashyizwe kuri 37 ° C icyumweru, ikomeza ibirenze90%ibikorwa;
◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.
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ByihuseEᵀᴹᵀᴹ Igihe nyacyo PCR Kit-Taqman
◮Sisitemu idasanzwe ya PCR yogukora ikora 2 × ByihuseTMPCR nyayo ivanze-Taqman irahuza cyane.
◮Gushyushya-gutangira Foregene HS Taq Polymerase ifite ubushobozi bwo kongera imbaraga, ibyiyumvo byiyongera, hamwe nuburyo bwihariye bwo kwongera.
◮2 × ByihuseTMReal PCR ivanze -Taqman ikoresha sisitemu idasanzwe yatunganijwe na Foregene kugirango itezimbere ibyiyumvo byihariye hamwe nubushakashatsi bwihariye bwo gutahura iperereza, bishobora gukoreshwa muri genotyping no gukoporora umubare ugereranya no kubona ibisubizo nyabyo.
◮Ibicuruzwa bizana irangi ryimbere rya ROX, rishobora gukoreshwa mugukuraho ibimenyetso byerekana ibimenyetso hamwe nikosa ryibimenyetso hagati yamariba, byorohereza abakiriya gukoresha muburyo butandukanye bwibikoresho bya PCR.
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Terefone
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