RT Byoroshyeᵀᴹ I Master Premix kumurongo wambere cDNA synthesis
Ibisobanuro:
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 25 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 ℃,kandi ifite imikorere myiza yo guhindura inyandiko.
- Ndetse na RNA yanduye (inzoga zigera kuri 45%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
Ibisobanuro
FOREGENE's 2 × RT BiroroshyeTMKuvanga birashobora gushushanya umurongo wa mbere wa cDNA ku bushyuhe buri hejuru ya 50 ° C, ibyo bikaba bifasha guhindura reaction ya reaction ya reaction ya kabiri ya RNA inyandikorugero.
RNA yasukuwe muri laboratoire ikubiyemo inzoga n’ibisigazwa by’umunyu wa guanidine, bifite imbaraga zikomeye zo kubuza kwandukura inyandiko zinyuranye, bikavamo ingaruka zidashimishije zinyuranye cyangwa uburyo bwo kwandukura buke.FOREGENE 2 × RT BiroroshyeTMImvange yerekana kwihanganira cyane inzoga n'umunyu wa guanidine, kwihanganira inzoga nyinshi mu ngero za RNA ni 45%, naho kwihanganira umunyu wa guanidine ni 750mM.Nubwo RNA yaba idafite isuku, reaction ya transcript irashobora gukorwa hamwe na FORREGENE 2 × RT ivanze.Sisitemu idasanzwe ya reaction ituma reaction ya RT yoroshye, byihuse kandi ikora neza, kandi synthesis yumurongo wambere cDNA irashobora kurangira muminota 25.
Ibicuruzwa birashobora gukoreshwa bifatanije na Foregene ya fluorescent yibicuruzwa byuzuye Igihe nyacyo PCR ByoroshyeTMkubona ibisubizo byujuje ubuziranenge ibisubizo.
Ibisobanuro
| RT BiroroshyeTMI. Master Premix kumurongo wambere-cDNA synthesis | ||
| Ibirimo | RT-01011 | RT-01012 |
| Inshuro 25 (sisitemu 20μl) | Inshuro 100 (sisitemu ya 20μl) | |
| 2 × RT EasyTM ivanze | 0.25ml | 1ml |
| Primer Primer (50μM) | 50μl | 200μl |
| Oligo (dT) 18 Primer (50μM) | 50μl | 200μl |
| RNase Yubusa ddH2O | 1.7ml | 1.7ml |
| Igitabo gikubiyemo amabwiriza | Igice 1 | Igice 1 |
Ibiranga ibyiza
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 25 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 and, kandi ifite imikorere myiza yo kwandukura.
- Ndetse na RNA yanduye (inzoga zigera kuri 45%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
Porogaramu
- RT-PCR isanzwe
- Synthesis ya cDNA yo gukoroniza no gukora ubushakashatsi
- Isesengura ryuburyo bwa primer uburyo bwo gutangiza inyandiko
- ISOKO (amplification yihuse ya cDNA irangira)
- Kwiyongera kwa RNA kumurongo
- Ikimenyetso
Urujya n'uruza rw'akazi
Ububiko nubuzima bwiza
1.Imiterere yo gutwara abantu
Inzira yose yubushyuhe bwo hasi yubusanduku bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ℃.
2. Ububiko
RT BiroroshyeTMNabitswe kuri -20 ° C.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20 ° C ako kanya ukimara kuyakira.Niba uburyo bwo kubika bukwiye, ibicuruzwa ntibishobora gutesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
