HER2 / CSP17 Amabara abiri
Ibisobanuro:
Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.
Ibisobanuro
Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| 5Ibizamini | 10Ibizamini | 20Ibizamini | |
| HER2 / CSP17 Amabara abiri | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane neza ubwiyongere bwa gene ya HER2 mu bice bya kanseri y'ibere ya kanseri y'umuntu yashyizwe hamwe na 10% itagira aho ibogamiye kandi yashyizwe muri paraffine.
Kugaragaza ibisabwa
Ibigereranirizo byari paraffine yashyizwemo ibice bya kanseri y'ibere byemejwe no gusuzuma indwara.
Mugutegura ibice bya paraffin, tissue nshya igomba gushyirwaho muri 10% itabogamye ya buffer formalin igisubizo cyamasaha 6 mugihe cyisaha 1;ubunini bw'igice bugomba kuba hagati ya 3 mm na 5 mm;ibishashara cyangwa ibice birashobora kugumaho amezi 12 nyuma yo gukama mubushyuhe bwicyumba.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
