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RT Easyᵀᴹ II Master Premix kumurongo wambere wa cDNA synthesis kumwanya nyawo PCR
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 20 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 and, kandi ifite imikorere myiza yo kwandukura.
- Ndetse na RNA yanduye (inzoga zigera kuri 60%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
- 2 × RT CYANGWA-ByoroshyeTM Kuvanga byongeweho uburyo bwiza bwo guhindura inyandiko (Primer Primer, Oligo (dT)18 Primer).
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