Amakuru yinganda
-
Witondere amakuru akurikira kugirango wikubye kabiri intsinzi ya RT-qPCR!
RT-qPCR nubushakashatsi bwibanze bwibinyabuzima bya molekuline, kandi buri wese agomba kubimenyera.Harimo cyane cyane intambwe eshatu: Gukuramo RNA, guhinduranya inyandiko muri cDNA, hamwe na PCR yibihe nyabyo PCR.Ntabwo bifasha, bigenda bite?Birashoboka ko hari ikibazo w ...Soma byinshi -
Gukuramo RNA, 260/230 ni hasi cyane, kandi ct agaciro ka pcr ikora ni hejuru cyane.Nigute wabikemura?
Umubare muto wa A260 / A230 ubusanzwe uterwa numwanda ufite uburebure ntarengwa bwo kwinjiza kuri 230nm.Reka turebe icyo ibyo byanduye birimo: Ibihumanya bisanzwe Absorption wavelength Ikigereranyo cya Protein ~ 230nm na 280nm Kugabanuka icyarimwe kugabanya A 260 / A 280 na A 260 / A ...Soma byinshi -
Fluorescent yuzuye PCR (qPCR) - igishushanyo mbonera
Mubigeragezo bya qPCR, primer igishushanyo nacyo gihuza cyane.Niba primers ikwiye cyangwa idakwiye bifitanye isano ya hafi niba imikorere ya amplification igera ku gipimo, niba ibicuruzwa byongerewe umwihariko, kandi niba ibisubizo byubushakashatsi bihari.Nigute rero gukora ...Soma byinshi -
Inyongera zisanzwe mubitekerezo bya PCR nimirimo yabyo
Nizera ko buriwese azahora ahura nibibazo nkibi cyangwa mugihe akora reaction ya PCR, ariko ibyinshi muribyo bishobora gushyirwa mubibazo bibiri byingenzi: Kwiyongera gukabije kwicyitegererezo cya gene (amplification);Byinshi cyane bitagenewe gene amplification.Gukoresha inyongeramusaruro nimwe murwego rusanzwe ...Soma byinshi -
Incamake yimiti 45 ivura gene yagurishijwe kwisi yose
Imirasire nigice cyibanze kigenga imiterere.Usibye ingirabuzimafatizo za virusi zimwe na zimwe, zigizwe na RNA, ingirabuzima fatizo zigizwe na ADN.Indwara nyinshi z’ibinyabuzima ziterwa n’imikoranire hagati ya gen n'ibidukikije.Ubuvuzi bwa Gene bushobora gukiza o ...Soma byinshi -
Mubihe byo kuvura RNA, ninde ushobora guhinduka "umukunzi mushya" winganda |Ibarura rya buri mwaka
Inkomoko: WuXi AppTec Mu myaka yashize, urwego rwo kuvura RNA rwerekanye ibintu biturika- mu myaka 5 ishize yonyine, imiti 11 ya RNA yemejwe na FDA, ndetse uyu mubare urenze umubare w’ubuvuzi bwa RNA bwemejwe mbere!Ugereranije nubuvuzi gakondo, RNA ivura ca ...Soma byinshi -
PCR isanzwe, fluorescent PCR, PCR ya digitale;gusesengura ibyiza n'ibibi!
Ibintu byinshi byavumbuwe mu mateka yubuhanga bwo gutahura mubitekerezo byanjye ni tekinoroji ikingira indwara ishingiye ku ihame rya antigen-antibody yihariye ihuza, ikoranabuhanga rya PCR hamwe n’ikoranabuhanga rikurikirana.Uyu munsi tuzavuga kubijyanye n'ikoranabuhanga rya PCR.Ukurikije t ...Soma byinshi -
Incamake | Kuva muri Kanama 2022, imiti 41 yo kuvura gene yatangijwe ku isi.
Vuba aha, imiti itatu yo kuvura gene yemerewe kwamamaza, aribyo: (1) Ku ya 21 Nyakanga 2022, PTC Therapeutics, Inc.Soma byinshi -
Ibihe byashize nubu bya CACLP
Kuva ku ya 26 kugeza ku ya 28 Ukwakira 2022, umunsi mukuru mushya w’ubuvuzi bw’ubushakashatsi no mu gusuzuma indwara ya vitro - Ishyirahamwe rya 19 ry’Ubushinwa ry’Ubushinwa ry’ubuvuzi bwa Laboratoire Y’imurikagurisha ku nshuro ya kabiri Ubushinwa IVD bwo gutanga amasoko (CISCE) bwabereye mu kigo mpuzamahanga cya Nanchang Greenland.FOREGENE Co ...Soma byinshi -
Imashini ya PCR | Urumva rwose?
Imashini ya PCR | Urumva rwose?Ikoranabuhanga rya PCR ryegukanye igihembo cyitiriwe Nobel Mu 1993, umuhanga w’umunyamerika Mulis yahawe igihembo cyitiriwe Nobel muri Chimie, kandi ibyo yagezeho ni uguhimba ikoranabuhanga rya PCR.Ubumaji bwa tekinoroji ya PCR buri mubiranga bikurikira: Icya mbere, ingano ya ADN kugeza ...Soma byinshi -
Ibitekerezo bishya bya Kamere: Gisesengura imiterere no guca ADN yuburyo bushya bwo guhindura gene ISRB
Mu myaka icumi ishize, tekinoroji yo guhindura gene ishingiye kuri CRISPR yateye imbere byihuse, kandi ikoreshwa neza mu kuvura indwara zikomoka kuri kanseri na kanseri mu bigeragezo by’amavuriro.Muri icyo gihe, abahanga ku isi bahora bakoresha ibikoresho bishya hamwe no guhindura gen ...Soma byinshi -
Ubuvuzi bwa Gene, "gukanguka" rwose ugutwi
Kunanirwa kwumva (HL) nindwara ikunze kwibasira ubumuga bwabantu.Mu bihugu byateye imbere, hafi 80% by’abana bafite ubumuga bwo kutumva mbere y’abana biterwa n’impamvu zishingiye ku ngirabuzima fatizo.Ikigaragara cyane ni inenge ya gene imwe (nkuko bigaragara ku gishushanyo 1), ihinduka rya gene 124 ryagaragaye ko rifitanye isano wi ...Soma byinshi
