PCR Isukura Kit PCR Isukura Sisitemu ya Ultra-yera
Ibisobanuro:
Byihuse usukure kandi ubone ibice bya ADN byujuje ubuziranenge muri sisitemu ya PCR;niba ushaka gutandukana no kubona ibice bya ADN bya ADN, nyamuneka hitamo ibikoresho byo kugarura gel.
Range Urwego runini rwo kugarura ADN: Ibice bya ADN bigufi nka 60bp kandi binini nka 10kb birashobora kugarurwa.
Performance Gukora neza: Gukora neza muri rusange birenze 80%, kandi hejuru irashobora kugera hejuru ya 95%.
Speed Umuvuduko wihuse:Biroroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15 kandi primer dimer irashobora gukurwaho bitabaye ngombwa gukira gel.
◮ S.umutekano: Nta gukuramo organic reagent bisabwa.
Quality Ubwiza bwo hejuru: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.
Ibisobanuro
Iki gikoresho gikoresha spin inkingi hamwe na formula yakozwe na societe yacu, irashobora kugarura neza ibice bya ADN bifite isuku nyinshi muri sisitemu ya PCR.Inkingi yo kweza irashobora guhuza ADN neza, kandi igahuzwa na formula idasanzwe reagent kugirango ikureho primer dimer itabanje gukira gel.
Ibikoresho bigarura ibice byinshi bya ADN.Mubihe bisanzwe, ibice bya ADN bigufi nka 60bp birashobora kugarurwa.Nibura 30μl ya eluent irashobora gukoreshwa kugirango wongere ubunini bwa ADN yagaruwe.
Igikoresho kiroroshye gukora kandi gifite intambwe nke.Irakeneye gusa centrifugation kugirango itunganyirize icyarimwe icyarimwe, kandi ibice byinshi bya ADN byagaruwe neza birashobora kuboneka muminota 15.
Ibisobanuro
50 Imyiteguro, 100Itegurwa, 250Itegurwa
Ibigize ibikoresho
| Buffer BD * |
| Buffer BD-S |
| Buffer WB1 |
| Buffer EB |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Ibice byinshi byo kugarura ADN: ibice bya ADN bigufi nka 60bp kandi binini nka 10kb birashobora kugarurwa.
-Ibikorwa byiza byo gukira: Ubushobozi bwo gukira burenze 80%, kandi hejuru irashobora kugera kuri 95%.
-Umuvuduko wihuse: Byoroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15 kandi primer dimer irashobora gukurwaho bitabaye ngombwa gukira gel.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.
Porogaramu
Iki gikoresho kibereye kugarura ibice bya ADN (60bp-10kb) mubicuruzwa bya PCR.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
