Ubushinwa Budafite ADN Yigunga Ibikoresho Gukora no Gutanga |Foregene

Akagari ka ADN kitagira akagari

Utugingo ngengabuzima ADN Yigunze Igikoresho cyerekanwe

Ibisobanuro:

Kwigunga kwa cfDNA uhereye kuri plasma

Umubare Cataloge TQ01BT0050, TQ01BT0100

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Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

ADN idafite selile (cfDNA) ni agace ka cfDNA isohoka mu ngirabuzimafatizo mu maraso ishobora gutwara amakuru y’ibinyabuzima kuvaibibyimba, indwara ziterwa na virusi.Isesengura rya cfDNA ritanga ibimenyetso byingenzi byo gusuzuma, gutahura no gukurikirana progres zindwarasion.Kugeza ubu, iryo koranabuhanga ryakoreshejwe cyane mu bijyanye no kumenya ihinduka ry’ibibyimba, kuyobora ibiyobyabwenge no guhanura,gutahura indwara ya virusi no kwipimisha mbere yo kubyara.

Acide nucleic yo mu rwego rwo hejuru niyo garanti yubushakashatsi bwa genomics.Foregene ikoresha tekinoroji ya superparamagneticguha abayikoresha igisubizo cyoroshye cya nucleic acide ikemura byoroshye kugera kuri acide nucleic aside ikuramo bikekwibanda.Nkuko byagenzuwe n’ibihumbi by’ubuvuzi, iki gicuruzwa gishobora gukuramo cfDNA yo mu rwego rwo hejuru mu mazi adafite selilenka plasma nshya cyangwa ikonje, serumu, pleural fluid, asite na cerebrospinal fluid kubushakashatsi bwa siyanse nubuvuzi muri vitro-kwisuzumishaKoresha.

Foregene Cell- idafite ADN Yigunga Igikoresho cyagenewe gutandukanya ADN idafite ingirabuzimafatizo (cfDNA) na plasma yumuntu, serumu, amazi yumubiri, cyangwa inkari.Igikoresho kirashobora kwihuta kandi byoroshye gutandukanya cfDNA mubyiciro bitatu: lysis / guhambira, gukaraba no gukuraho.Ikoresha amashanyarazi yihariye ya superparamagnetic hamwe na sisitemu idasanzwe ya lysis / binding kugirango igarure acide nucleique ikwirakwizwa na hydrogène ihuza imbaraga na electrostatike, itarinze poroteyine nibindi byanduye.Birakwiriye cyane-kwinjiza ibicuruzwa byikora bikurura imirimo- sitasiyo mugutegura isesengura iryo ariryo ryose ukoresheje PCR cyangwa NGS.

Ibikoresho n'ibikoresho

Imbonerahamwe 1 Foregene Akazu- kitagira ADN Yigunze

Contents	Reagent	Umubare(25 T)	Umubare(50 T)	Umubare(100 T)	Storage
Agasanduku1	Buffer AL	Icupa rya mLx1 42	Icupa rya mLx1 89	189 icupa rya mLx1	1 0- 30 ° C.
	Buffer AW1	Icupa rya mLx1 38	Icupa rya mLx1 83	165 mLx1 icupa	1 0- 30 ° C.
	Buffer AW2	Icupa rya mLx1	Icupa rya mLx1 33	Icupa rya mLx1 66	1 0- 30 ° C.
	Gukuraho	Icupa rya mLx1	Icupa rya mLx1	Icupa rya mLx1	1 0- 30 ° C.
Agasanduku2	Amasaro ya rukuruzi A.	3 .2 icupa rya mLx1	6 .5 mLx1 icupa	Icupa rya mLx1	2 - 8 ° C.
Agasanduku2	Proteinase K.	Icupa rya mLx1	10 .5 mLx1 icupa	Icupa rya mLx1	2 - 8 ° C.

Inyandiko:

[1] Ntukavange ibice biva mubice bitandukanye.

[2] Buffer AL irashobora gukora imvura, itagira ingaruka kumikorere yayo.Niba imvura igaragara, nyamuneka shyira icupa rya reagent mu bwogero bw’amazi 56 ° C muminota 1 0 - 20 kugeza igihe imvura iguye.Noneho vanga neza mbere yo gukoresha.

[3] Ntugahagarike amasaro ya Magnetique A.