Ikirangantego cya ADN Micro Kit Micro Genomic ADN Mini Kit
Ibisobanuro:
Sukura vuba ingero ntoya kugirango ubone ADN nziza-nziza.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Iki gikoresho gikoresha ADN-Inkingi yonyine ishobora guhuza ADN, protease nshya-ya Foregene Protease hamwe na sisitemu yihariye ya buffer, ishobora gukuramo neza ingero zitandukanye z’ibinyabuzima, urugero nk’amaraso, selile, n’inyamaswa mu minota 20-80.Tissue, amaraso yumye, ipamba ya clinique, imisatsi, nibindi, byera kugirango bibone ADN yibanze cyane.
Ibisobanuro
50 Imyiteguro
Ibigize ibikoresho
| Buffer ST1 |
| Buffer ST2 * |
| Buffer PW * |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyubushakashatsi, bikabuza laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease ifite ibikorwa byinshi kuruta protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
-Ibyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
-Micro-elution sisitemu: Irashobora kongera ubunini bwa ADN ya genomic, ikaba yorohereza gutahura cyangwa kugerageza.
Porogaramu
Birakwiriye kwezwa ingero zikurikira za ADN ya genomic: inyama zinyamanswa nshya cyangwa zikonje, inyama nshya cyangwa anticoagulated cryopreserved marike yamaraso yose, ibibara byamaraso yumye, selile yumuco, uduce duto twa microsection, imisatsi, kwoza umunwa, nibindi.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);ibikorwa byayo no gutuza bizaba byiza iyo bibitswe kuri 4°C, birasabwa rero kubika kuri4 ° C, ibuka kutayigumana kuri -20 ° C.
