FFPE ADN Yigunze Igikoresho cyo gukuramo ADN yo gukuramo ibikoresho bya FFPE
Ibisobanuro:
Byihuse usukure ADN yo mu rwego rwo hejuru ivuye mu bice byashyizwemo ibice nka para ffi n ibice na para ffi n bice.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Iki gikoresho cyahariwe gukuramo no kweza ADN yo mu rwego rwohejuru ya ADN ikomoka kuri tissue ya formaldehyde cyangwa tissue ya paraffin.
ADN ya genomic ya sample ya paraffin yashyizwemo izangirika bitewe nigihe cyo kubika cyangwa ibidukikije, bikagorana gukuramo ADN genomic.Iki gikoresho gikoresha formulaire idasanzwe, ikwiranye no gukuramo ADN genomic muri paraffin yashizwemo ingero hamwe nigipimo kinini cyo gukuramo.Inzira yose ntikeneye gukoresha ubumara bwa fenol, chloroform cyangwa imvura ya isopropanol.
Iki gikoresho gikoresha uruziga rushobora guhuza ADN, igikoresho gishya cya Foregene Protease Plus, hamwe na sisitemu yihariye, ishobora gukuramo ADN ya genoside yo mu rwego rwo hejuru mu masaha 2-5.
Ibisobanuro
50 Imyiteguro, 100 Itegura, 250 Itegura
Ibigize ibikoresho
| Buffer FL1 |
| Buffer FL2 * |
| Buffer PW * |
| Buffer WB |
| Buffer EB |
| Foregene Protease Yongeyeho |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kuruta protease isa, kandi igogora ingero za tissue byihuse;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira mumasaha 2-5.
-Ibyoroshye: Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4°C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Irakwiriye gukuramo no kweza ADN ya genomique ikomoka kuri forode ya fordehide hamwe na paraffin yashizwemo na tissue.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease Plus igisubizo gifite formula idasanzwe ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);birakora cyane kandi bihamye iyo bibitswe kuri 4 ° C, birasabwa rero kubibika kuri 4 ° C, ibuka kutabika kuri -20 ° C.
