EndoFree Maxi Plasmid Kit (Inkingi izunguruka)
Ibisobanuro:
Ibikorwa byose byo gukuramo bifata isaha 1 gusa, byemeza imikorere yoroshye kandi yihuse.
Ibisobanuro
EndoFree Maxi Plasmid Kit ikoresha tekinike idasanzwe ya silika membrane adsorption tekinike kugirango ihuze neza na ADN ya plasmide.Muguhuza Endotoxin idasanzwe yo gukuraho Buffer P4 na EndoFree Maxi Filtration Inkingi, endotoxine, proteyine nibindi byanduye birashobora kuvaho neza.Ibikorwa byose byo gukuramo bifata isaha 1 gusa, byemeza imikorere yoroshye kandi yihuse.
Indwara ya bagiteri isabwa kuri buri myitozo: Kuri plasmid-yandukuwe cyane, birasabwa gukoresha ml 100 yibitangazamakuru byumuco wa bagiteri, kandi umusaruro uva muri rusange hafi 500-1500 μg.Kuri plasmid nkeya, 200 ml itangazamakuru ryumuco wa bagiteri rirasabwa kubyara hafi 200-600 μg plasmide.
Ibigize ibikoresho
| Ibicuruzwa Ibigize | D909-EPre 10 itegura） |
| Buffer BL | 30 ml |
| Buffer P1 | 100 ml |
| Buffer P2 | 100 ml |
| Buffer P4 | 100 ml |
| Buffer DW2 | 44 ml |
| Buffer EB | 30 ml |
| RNase A (10 mg / ml) | 1 ml |
| ADN Yuzuye Inkingi | 10 pc |
| Byiza Maxi Muyunguruzi Syringe | 10 pc |
| 50 ml Ikusanyirizo | 10 pc |
Kumenya Kwibanda kwa ADN ya Plasmid hamwe nubuziranenge
Ubuziranenge hamwe nubushuhe bwa ADN yakuwe muri plasmide irashobora gutahurwa na agarose gel electrophorei na ultraviolet spectrophotometer.Agaciro ka OD260 ka 1 gahuye na 50 μg / ml ya ADN ya kabiri.Agaciro ka OD260 / OD280 ka ADN ya plasmide isukuye muri rusange iri hagati ya 1.7-1.9.ADN ya plasmid isukuye irashobora gukoreshwa muburyo butaziguye mubigeragezo bisabwa cyane, nko kwanduza selile cyangwa mubushakashatsi bwa vivo.
Porogaramu
ADN ya plasmid yakuwe niki gikoresho irashobora gukoreshwa mubikorwa bitandukanye bisanzwe, harimo igogorwa ryimisemburo, PCR, ikurikiranye, guhuza, guhinduka kimwe no kwanduza ubwoko butandukanye.
Ububiko hamwe nubuzima bwa Shelf
EndoFree Maxi Plasmid Kit irashobora kubikwa mubushyuhe bwicyumba (15-25 ° C) mugihe cyumye mugihe cyamezi 12.Ongeramo RNase A kuri Buffer P1 mbere yo gukoresha bwa mbere, vanga neza kandi ubike kuri 2-8 ° C.Kuvanga birashobora kubikwa neza mumezi arenze 6.
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