Intebe ya ADN Yigunze Igikoresho cya ADN Ikuramo Ibikoresho byoza
Ibisobanuro:
Byihuse usukure kandi ubone ADN ya genomic yo mu rwego rwo hejuru uhereye ku ntebe zitandukanye.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Iki gikoresho gitanga uburyo bwihuse kandi bworoshye bwo kuvana ADN genomic mumasoko atandukanye yubutaka.Hano hari umubare munini wa inhibitor nka acide humic na ion zicyitegererezo mubutaka.Nubwo ibyo bintu biboneka muke muri ADN isukuye, bizagira ingaruka kumyitwarire yo hasi, nka PCR hamwe no kubuza enzyme igogorwa.Kubwibyo, urufunguzo rwo kweza ubutaka ADN nuburyo bwo gukuraho neza inhibitori mu butaka.
Iki gikoresho gikoresha ADN-Inkingi yonyine ishobora guhuza cyane na ADN, protease nshya ya Foregene Protease hamwe na sisitemu yihariye ya buffer, ishobora kuvanaho neza inibitori zitandukanye mu butaka zidakuweho ibinyabuzima cyangwa Ethanol hamwe n’imvura ya isopropanol.Gukuramo ADN mubutaka bwubutaka birashobora kurangira muminota 90.
Ibisobanuro
25 Imyiteguro, 50 Itegura, 100 Itegura, 250 Itegura
Ibigize ibikoresho
| Buffer SL1 |
| Buffer SL2 |
| Buffer SL3 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyubushakashatsi, bikabuza laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN ya fecal birashobora kurangira muminota 40.
-Ibyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Birakwiriye kweza ADN genomic duhereye ku ngero zikurikira: abantu bashya cyangwa bakonje, inka, inkoko, imbeba nizindi ntangarugero.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kirekire (amezi 3), ibikorwa byayo nibituza bizaba byiza iyo bibitswe kuri 4 ° C, bityo rero birasabwa kubibika kuri 4 ° C, wibuke kutabika kuri -20 ° C.
Ifu yumye ya Lysozyme ibikwa kuri -20 ° C, igisubizo cya Lysozyme cyateguwe kigabanyijemo uduce duto kandi kibikwa kuri -20 ° C.
