Imbuto y'ibihingwa by'Ubushinwa (binini kandi biciriritse) Direct PCR Plus Kit II-UNG (idafite ibikoresho by'icyitegererezo) Ihingura n'utanga isoko |Foregene

Imbuto y'ibihingwa (binini kandi biciriritse) Direct PCR Yongeyeho Kit II-UNG (idafite ibikoresho by'icyitegererezo)

Ibisobanuro:

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gicuruzwa gikoresha uburyo bwihariye bwa lysis reaction, bushobora kurekura byihuse ADN ya genomique yintangarugero yimbuto yibihingwa hamwe na polysaccharide nyinshi na polifenole (nk'imineke, ipamba, nibindi) kugirango ikoreshwe mubitekerezo bya PCR, bityo rero irakwiriye cyane cyane kwipimisha rinini.Kugirango uhuze ibikenewe mu bizamini bitandukanye byo gutahura, iki gikoresho kirashobora gukoresha ubwoko butandukanye bwintangarugero (nkimbuto zose, gukata uduce duto, cyangwa ingero zubutaka bwimbuto nyinshi) mubushakashatsi.Muri byo, kuburugero rwimbuto rugikeneye kumera, urashobora guhitamo guca uduce duto duto (1-5mg) usibye insoro zimbuto kugirango zigerageze;kubushakashatsi busaba icyitegererezo no kwipimisha mumubare munini wintangarugero, urashobora guhitamo kuvanga ingero nyinshi hanyuma ukazihuza.Nyuma yo gusya mubice bifite diameter ya 0.5mm, ubushakashatsi burashobora gukorwa (byibuze 0.1% byurugero rwicyitegererezo birashobora kuboneka).

Ibisobanuro

50T, 200T, 500T, 2000T

Ibigize ibikoresho

Imbuto y'ibihingwa Direct PCR Yongeyeho Kit II-UNG Polysaccharide polifenol imbuto yimbuto (nini nini nini) itaziguye PCR kit-UNG
Ibigize ibikoresho		TP-0318T	TP-03181	TP-03182	TP-03183
Ibigize ibikoresho		50 T.	200 T.	500 T.	2000 T.
Igice I.	Buffer SSP1	24ml	96ml	120ml × 2	480ml × 2
	Buffer SP2	3ml	10ml	25ml	100ml
	6 × ADN Yipakurura Buffer	1.5ml	1.5ml	1.5ml	1.5ml × 4
Igice cya II	Buffer SSP2	8ml	32ml	80ml	80ml × 4
Igice cya II	2 × Imbuto PCR EasyTM ivanze	500μl	1ml × 2	1.7ml × 3	1.7ml × 12
Igitabo		1	1	1	1

Ibiranga ibyiza

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Kurwanya umwanda PCR sisitemu 2 × Imbuto PCR Byoroshye^TM Kuvanga (UNG), bishobora gukuraho neza umwanda uterwa nibicuruzwa bya PCR no kwemeza umwihariko nukuri kwa amplification.