Amaraso SuperDirectᵀᴹ PCR Kit (UNG) -Heparin Amaraso Direct PCR Master mix ya Genotyping yamaraso
Ibisobanuro:
Koresha mu buryo butaziguye heparin anticoagulated maraso yose nkicyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.
◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.
◮2 × ByoroshyeTMKuvanga (UNG) -Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR
◮2 × ByoroshyeTMKuvanga (UNG), kurandura burundu umwanda uterwa nibicuruzwa bya PCR, kandi urebe neza umwihariko nukuri kwa amplification.
Ibisobanuro
200 × 20μl rxns, 2000 × 20μl rxns
2 × ByoroshyeTMKuvanga (UNG) -Heparin: Ukurikije 2 × SuperEasyTMKuvanga-Heparin, dUTP ikoreshwa mu mwanya wa dTTP, kandi enzyme ya UNG ishobora gutesha agaciro inyandikorugero ya dUTP yongewemo icyarimwe, ishobora gukumira neza kwanduza ibicuruzwa byongera PCR.Harimo D-Taq ADN Polymerase yahinduwe, DNTPs, MgCl2, buffer reaction, PCR yongerera imbaraga, optimizer na stabilisateur, nibindi.2O kuri 2 × SuperEasyTM ivanze (UNG) -Heparin kugirango ikoreshwe mubitekerezo bya PCR.
6 Buffer Yipakurura ADN: Buffer Yipakurura ntabwo irimo SDS.Birasabwa gukoresha 6 × ADN Yipakurura Buffer yashyizwemo nibikoresho mugihe ukora agarose gel electrophorei kugirango ubone ibisubizo byiza bya electrophorei.Ntukoreshe Loading Buffer irimo SDS, bitabaye ibyo hazaba umurizo munini wumucyo mwinshi mumurongo mugihe cya electrophoreis, bizagira ingaruka kubisubizo byubushakashatsi.
Ibigize ibikoresho
| 2 × ByoroshyeTMKuvanga (UNG) -Heparin |
| 6 × ADN Yipakurura Buffer |
| Amabwiriza |
Ibiranga ibyiza
-Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.
-2 × ByoroshyeTMKuvanga (UNG) -Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
-Urugero rufunze byuzuye, ntabwo rero bikenewe guhangayikishwa no kwanduza icyitegererezo hamwe nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR 2 × SuperEasyTMKuvanga (UNG), kurandura burundu umwanda uterwa nibicuruzwa bya PCR, kandi urebe neza umwihariko nukuri kwa amplification.
Porogaramu
Yeguriwe kuyobora PCR imenyekanisha Heparin irwanya amaraso yose.(Harimo: amaraso yabantu, amaraso yimbeba, amaraso yinkoko, amaraso yinyoni, amaraso yinka, amaraso yimbwa, nibindi)
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Inzira yose yubushyuhe bwo hasi yubusanduku bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ℃.
2 × ByoroshyeTMKuvanga (UNG) -Heparin, iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ° C kugirango ibike igihe gito (koresha muminsi 10).
6 × ADN Yipakurura Buffer, irashobora kubikwa kuri 4 ° C cyangwa -20 ° C mugihe kirekire.
