Amaraso SuperDirectᵀᴹ PCR Kit-Heparin Amaraso Direct PCR Master mix ya Genotyping yamaraso
Ibisobanuro:
Koresha mu buryo butaziguye heparin anticoagulated maraso yose nkicyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.
◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.
◮2 × ByoroshyeTMKuvanga-Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.
Ibisobanuro
200 × 20μl rxns, 2000 × 20μl rxns
2 × ByoroshyeTMKuvanga-Heparin ： Irimo Polymerase ya D-Taq yahinduwe idasanzwe, DNTPs, MgCl2, buffer reaction, kongera imbaraga za PCR, optimizer, stabilisateur, nibindi.2O kuri 2 × SuperEasy ihuyeTMKuvanga-Heparin kugirango ikoreshwe muri reaction ya PCR.
6 Buffer Yipakurura ADN Buff Buffer Yipakurura ntabwo irimo SDS.Birasabwa gukoresha 6 × ADN Yipakurura Buffer yashyizwemo nibikoresho mugihe ukora agarose gel electrophorei kugirango ubone ibisubizo byiza bya electrophorei.Ntukoreshe Loading Buffer irimo SDS, bitabaye ibyo hazaba umurizo munini wumucyo mwinshi mumurongo mugihe cya electrophoreis, bizagira ingaruka kubisubizo byubushakashatsi.
Ibigize ibikoresho
| 2 × SuperEasyTM Ivanga-Heparin |
| 6 × ADN Yipakurura Buffer |
| Amabwiriza |
Ibiranga ibyiza
-Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
-Uburyo bufite ubushobozi bukomeye bwo kongera imbaraga kandi burashobora gutahura neza ibice bigize ADN bigizwe na ADN mu maraso.
-2 × ByoroshyeTMKuvanga-Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
-Urugero rufunze byuzuye, ntabwo rero bikenewe guhangayikishwa no kwanduza icyitegererezo hamwe nibisubizo byiza bya PCR.
Porogaramu
Yeguriwe kuyobora PCR kumenya Heparin irwanya amaraso yose.(Harimo: amaraso yabantu, amaraso yimbeba, amaraso yinkoko, amaraso yinyoni, amaraso yinka, amaraso yimbwa, nibindi)
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho kigomba kubikwa kuri -20 ℃。
2 × ByoroshyeTMKuvanga-Heparin ， Niba ikoreshwa kenshi, irashobora kubikwa kuri 4 ° C kugirango ibike igihe gito (koresha muminsi 10).
6 Buffer Yipakurura ADN, Irashobora kubikwa kuri 4 ° C cyangwa -20 ° C igihe kirekire.
