Amaraso SuperDirectᵀᴹ PCR Kit (UNG) -EDTA Amaraso Direct PCR Master mix ya Genotyping yamaraso
Ibisobanuro:
Koresha mu buryo butaziguye EDTA irwanya amaraso yose nk'icyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.
◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.
◮2 × ByoroshyeTMKuvanga (UNG) -EDTA ifite kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR
◮2 × ByoroshyeTMKuvanga (UNG) -EDTA, ikuraho neza umwanda uterwa nibicuruzwa bya PCR, kandi ukemeza umwihariko nukuri kwa amplification.
Ibisobanuro
200 × 20μl rxns, 2000 × 20μl rxns
2 × ByoroshyeTMKuvanga (UNG) -EDTA: Ukurikije 2 × SuperEasyTMKuvanga-EDTA, dUTP ikoreshwa mu mwanya wa dTTP, kandi enzyme ya UNG ishobora gutesha agaciro inyandikorugero irimo dUTP yongewemo icyarimwe, ishobora gukumira neza kwanduza ibicuruzwa byongera PCR.Harimo D-Taq ADN Polymerase yahinduwe, DNTPs, MgCl2, buffer reaction, PCR yongerera imbaraga, optimizer na stabilisateur, nibindi.2O kuri 2 × SuperEasy ihuyeTMKuvanga (UNG) -EDTA kugirango ikoreshwe muri reaction ya PCR.
6 Buffer Yipakurura ADN Buff Buffer Yipakurura ntabwo irimo SDS.Birasabwa gukoresha 6 × ADN Yipakurura Buffer yashyizwemo nibikoresho mugihe ukora agarose gel electrophorei kugirango ubone ibisubizo byiza bya electrophorei.Ntukoreshe Loading Buffer irimo SDS, bitabaye ibyo hazaba umurizo munini wumucyo mwinshi mumurongo mugihe cya electrophoreis, bizagira ingaruka kubisubizo byubushakashatsi.
Ibigize ibikoresho
| 2 × ByoroshyeTMKuvanga (UNG) -EDTA |
| 6 × ADN Yipakurura Buffer |
| Amabwiriza |
Ibiranga ibyiza
- Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
-Uburyo bufite ubushobozi bukomeye bwo kongera imbaraga kandi burashobora gutahura neza ibice bigize ADN bigizwe na ADN mu maraso.
-2 × ByoroshyeTMKuvanga (UNG) -EDTA ifite kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
-Urugero rufunze byuzuye, ntabwo rero bikenewe guhangayikishwa no kwanduza icyitegererezo hamwe nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR 2 × SuperEasyTMKuvanga (UNG) -EDTA, ikuraho neza umwanda uterwa nibicuruzwa bya PCR, kandi ukemeza umwihariko nukuri kwa amplification.
Porogaramu
Yeguriwe kuyobora PCR imenyekanisha EDTA irwanya amaraso yose.(Harimo: amaraso yabantu, amaraso yimbeba, amaraso yinkoko, amaraso yinyoni, amaraso yinka, amaraso yimbwa, nibindi)
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho kibitswe kuri -20 ° C.
2 × ByoroshyeTMKuvanga (UNG) -EDTA ， Niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ° C kugirango ubike igihe gito (koresha muminsi 10).
6 Buffer Yipakurura ADN, Irashobora kubikwa kuri 4 ° C cyangwa -20 ° C igihe kirekire.
