Amaraso SuperDirectᵀᴹ PCR Kit-EDTA Amaraso Direct PCR Master Mix ya Genotyping yamaraso
Ibisobanuro:
Koresha mu buryo butaziguye EDTA irwanya amaraso yose nk'icyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.
◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.
◮2 × ByoroshyeTMKuvanga-EDTA Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.
Ibisobanuro
200 × 20μl rxns, 2000 × 20μl rxns
2 × ByoroshyeTMKuvanga-EDTA: Harimo Polymerase ya D-Taq yahinduwe bidasanzwe, dNTPs, MgCl2, reaction reaction, kongera imbaraga za PCR, optimizer na stabilisateur, nibindi.2O kuri 2 × SuperEasy ihuyeTMKuvanga-EDTA gukoreshwa muri reaction ya PCR.
6 Buffer Yipakurura ADN: Buffer Yipakurura ntabwo irimo SDS.Birasabwa gukoresha 6 × ADN Yipakurura Buffer yashyizwemo nibikoresho mugihe ukora agarose gel electrophorei kugirango ubone ibisubizo byiza bya electrophorei.Ntukoreshe Loading Buffer irimo SDS, bitabaye ibyo hazaba umurizo munini wumucyo mwinshi mumurongo mugihe cya electrophoreis, bizagira ingaruka kubisubizo byubushakashatsi.
Ibigize ibikoresho
| 2 × ByoroshyeTMKuvanga-EDTA |
| 6 × ADN Yipakurura Buffer |
| Amabwiriza |
Ibiranga ibyiza
-Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.
-Uburyo bufite ubushobozi bukomeye bwo kongera imbaraga kandi burashobora gutahura neza ibice bigize ADN bigizwe na ADN mu maraso.
-2 × ByoroshyeTMKuvanga-EDTA Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.
-Urugero rufunze byuzuye, ntabwo rero bikenewe guhangayikishwa no kwanduza icyitegererezo hamwe nibisubizo byiza bya PCR.
Porogaramu
Yeguriwe kuyobora PCR imenyekanisha EDTA irwanya amaraso yose.(Harimo: amaraso yabantu, amaraso yimbeba, amaraso yinkoko, amaraso yinyoni, amaraso yinka, amaraso yimbwa, nibindi)
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho kigomba kubikwa kuri -20 ° C.
2 × ByoroshyeTMKuvanga-EDTA, iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).
6 × ADN Yipakurura Buffer, irashobora kubikwa kuri 4 ° C cyangwa -20 ° C mugihe kirekire.
