Amaraso ADN Mini Kit
Ibisobanuro:
Sukura vuba ADN ya genomic yo mu rwego rwo hejuru mumaraso ya anticagulée (<1ml).
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Iki gikoresho gikoresha Foregene Protease Plus hamwe na sisitemu yihariye ya BL1 na BL2, ishobora gusya rwose urugero rwamaraso ya anticoagulant mugihe gito, bityo ikirinda kwangirika kwa ADN kurwego rwo hejuru kandi ikabona umubare munini wa ADN ya ADN.Sisitemu yihuse yo gutunganya amaraso hamwe nuburyo bworoshye bwa ADN-Gukora inkingi byoroshya cyane gukuramo genome yamaraso, bigatuma ADN genomic irashobora kuboneka mubwiza buhanitse kandi bwera muminota 40.
ADN yonyine ya silika gel membrane ikoreshwa muri centrifuge inkingi yiki gikoresho ni ubwoko bushya bwibintu byihariye muri sosiyete yacu, bishobora guhuza ADN neza kandi byumwihariko, kandi birashobora gukuraho RNA, umwanda, proteyine, ion nibindi bintu kama kama mungirabuzimafatizo ku rugero runini.Ibice bya ADN byabonetse ni binini, bifite isuku nyinshi, bihamye kandi byizewe.Ubushobozi ntarengwa bwo gutwara ADN-Inkingi gusa ni 80 μg ADN.ADN yabonetse irashobora gukoreshwa mubushakashatsi bwibinyabuzima bwa molekuline nko gusya enzyme, PCR, kuvanga amajyepfo no kubaka isomero.
Igikoresho kirashobora gutunganya amaraso agera kuri 1ml icyarimwe kugirango ubone urugero ruhagije rwa ADN ya genomique.
Ibisobanuro
| Amaraso ADN Mini Kit | |||
| Ibirimo | DE-05111 | DE-05112 | DE-05113 |
| 50 T. | 100 T. | 250 T. | |
| Buffer BL1 | 15 mL | 30 ml | 75 ml |
| Buffer BL2 * | 15 mL | 30 ml | 75 ml |
| Buffer DC | 100 mL | 200 ml | 500 ml |
| Buffer PW * | 25 mL | 50 ml | 125 ml |
| Buffer WB1 | 15 mL | 30 ml | 75 ml |
| Buffer EB | 10 mL | 20 ml | 50 ml |
| Foregene Protease Yongeyeho | 1.25 mL | 2,5 ml | 6.5 ml |
| ADN-Inkingi gusa | 50 | 100 | 250 |
| Igitabo | 1 | 1 | 1 |
*: Buffer BL2 na Buffer PW irimo umunyu utuje.Nyamuneka kwambara uturindantoki kandi ufate ingamba zo kubarinda mugihe ukora.
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue byihuse;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 40.
-Icyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, 4 ° C centrifugation cyangwa Ethanol imvura ya ADN ntabwo isabwa.
-Umutekano: Nta gukuramo organic reagent ikoreshwa.
-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Birakwiriye gukuramo no kweza ADN genomic mumaraso mashya cyangwa akonje.
Urujya n'uruza rw'akazi
Ububiko nubuzima bwiza
Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
Igisubizo cya Foregene Protease Plus gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);bizarushaho gukora kandi bihamye iyo bibitswe kuri 4 ° C, birasabwa rero kubibika kuri 4 ° C, ibuka kutabika kuri -20 ° C.
