Virusi RNA Yigunga Igikoresho cya RNA Isukura muri Plasma, Serumu, nizindi ngero
Ibisobanuro:
Injangwe.No.RE-02011/02014
Kugirango usukure virusi ya RNA muri plasma, serumu, umubiri utagira selile, umubiri ndengakamere.
Tandukanya vuba kandi usukure virusi ya RNA mubitegererezo nka plasma, serumu, amazi yumubiri utagira selile hamwe nindengakamere yumuco.
-Ntabwo ukeneye guhangayikishwa no kwangirika kwa RNA.Ibikoresho byose ni RNase-Ubuntu
-Icyoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba
-Byihuta-ibikorwa birashobora kurangira muminota 20
-Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA
-Umutekano-nta reagent ikoreshwa
-Ubushobozi bunini bwo gutunganya-bigera kuri 200μl byintangarugero birashobora gutunganywa buri gihe.
-Ubuziranenge bwo hejuru - RNA isukuye ni nziza cyane, idafite poroteyine n’indi mwanda, kandi irashobora guhura nuburyo butandukanye bwo kugerageza.
Ibisobanuro
Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza isuku nziza kandi nziza ya virusi ya RNA muburugero nka plasma, serumu, amazi adafite selile, hamwe numuco ndengakamere.Igikoresho kongeramo byumwihariko Linear Acrylamide, ishobora gufata byoroshye RNA nkeya mubitegererezo.RNA-Inkingi yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.
Ibikoresho byose ntabwo birimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer viRW1 na Buffer viRW2 birashobora kwemeza ko aside nucleic yabonetse yabonetse idafite proteyine, nuclease cyangwa iyindi myanda, ishobora gukoreshwa mu buryo butaziguye mu bushakashatsi bw’ibinyabuzima bwa molekile.
Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Ibigize ibikoresho
| Acrylamide |
| Buffer viRL |
| Buffer viRW1, Buffer viRW2 |
| Ubusa ddH2O |
| RNA-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Ntabwo ukeneye guhangayikishwa no kwangirika kwa RNA.Ibikoresho byose ni RNase-Ubuntu
-Icyoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba
-Byihuta-ibikorwa birashobora kurangira muminota 20
-Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA
-Umutekano-nta reagent ikoreshwa
-Ubushobozi bunini bwo gutunganya-bigera kuri 200μl byintangarugero birashobora gutunganywa buri gihe.
-Ubuziranenge bwo hejuru - RNA isukuye ni nziza cyane, idafite poroteyine n’indi mwanda, kandi irashobora guhura nuburyo butandukanye bwo kugerageza.
Ibipimo by'ibikoresho
Gusaba ibikoresho:
Irakwiriye gukuramo no kweza virusi ya RNA mu ngero nka plasma, serumu, umubiri utagira selile hamwe numuco ndengakamere.
Urujya n'uruza rw'akazi
Imiterere yo kubika
- Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃), cyangwa 2-8 ℃ mugihe kirekire.
-Umuti wa Acrylamide urashobora kubikwa mubushyuhe bwicyumba muminsi 7.Nyuma yo kwakira ibikoresho, nyamuneka fata igisubizo cya Linear Acrylamide hanyuma ubibike kuri -20 ℃.
-Nyuma yo kongeramo Linear Acrylamide kuri Buffer viRL, irashobora kubikwa kuri 2-8 ℃ kugeza kuri 48h.Nyamuneka koresha igisubizo gishya cyateguwe.
Imiyoboro yo gusesengura ibibazo
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Nta RNA ishobora gukururwa cyangwa umusaruro wa acide nucleic ni muke
Mubisanzwe hariho ibintu byinshi bigira ingaruka kumikorere yo gukira, nka: icyitegererezo cya RNA, uburyo bwo gukora, ingano ya elution, nibindi .。
Isesengura ry'impamvu zisanzwe ：
1.Kora ubwogero cyangwa ubushyuhe buke (4 ° C) centrifugation mugihe ukora.
Icyifuzo: Ubushyuhe bwicyumba (15-25 ° C) imikorere, ntuzigere woga urubura hamwe na centrifuge yubushyuhe buke.
2. Kubika icyitegererezo kidakwiye cyangwa ububiko bw'icyitegererezo igihe kirekire.
Icyifuzo: Bika ingero kuri -80 ° C cyangwa uhagarike muri azote yuzuye, kandi wirinde gukoresha inshuro nyinshi;gerageza gukoresha ingero zegeranijwe zivuye muri RNA.
3.Icyitegererezo kidahagije
Icyifuzo: Nyamuneka menya neza ko icyitegererezo nigisubizo cyakazi (Linear Acrylamide) byavanze neza kandi bigashyirwa muminota 10 mubushyuhe bwicyumba (15-25 ° C)
4.Umuhanga wongeyeho nabi
Icyifuzo: Menya neza ko ddH2O yubusa ya RNase yongewe hagati ya membrane yinkingi yo kweza
5.Ubunini budakwiye bwa anhydrous ethanol muri Buffer viRW2
Igitekerezo: Nyamuneka kurikiza amabwiriza, ongeramo ingano yukuri ya anhydrous ethanol kuri Buffer viRW2 hanyuma ubivange neza mbere yo gukoresha ibikoresho。
6.Ikoreshwa ry'icyitegererezo kidakwiye.
Igitekerezo: 200µl yicyitegererezo kuri 500μl ya Buffer viRL.Ingano yikitegererezo irenze izagabanya igipimo cyo gukuramo RNA.
7.Ijwi ridakwiriye cyangwa gusohora kutuzuye.
Igitekerezo: Ingano nziza yinkingi yo kweza ni 30-50μl;niba ingaruka zo gukuraho zidashimishije, birasabwa kongeramo ubushyuhe bwa RNase-Ubusa ddH2O hanyuma wongere umwanya ushira mubushyuhe bwicyumba, nka 5-10min
8.Inkingi yo kweza ifite ibisigisigi bya Ethanol nyuma yo koza muri Buffer viRW2.
Igitekerezo: Niba Ethanol ikomeje kuguma nyuma yo koza muri Buffer viRW2 hamwe na centrifugation yubusa ya 2min, inkingi yo kweza irashobora gusigara mubushyuhe bwicyumba cya 5min nyuma ya centrifugation yubusa kugirango ikureho Ethanol isigaye.
Kwangirika kwa molekile ya RNA isukuye
Ubwiza bwa RNA yatunganijwe bujyanye nibintu nko kubika icyitegererezo, kwanduza RNase, no gukora.
Isesengura ry'impamvu zisanzwe ：
1.Icyitegererezo cyegeranijwe nticyabitswe mugihe.
Igitekerezo: Niba icyitegererezo kidakoreshejwe mugihe cyo gukusanya, nyamuneka ubike kuri -80 ℃ cyangwa azote yuzuye.Kubikuramo molekile ya RNA, gerageza gukoresha ingero zegeranijwe igihe cyose bishoboka.
2.Ingero zegeranijwe zarakonje kandi zishonga inshuro nyinshi.
Igitekerezo: Irinde gukonjesha no gukonjesha inshuro nyinshi (bitarenze rimwe) mugihe cyo gukusanya no kubika, bitabaye ibyo umusaruro wa acide nucleique uzagabanuka.
3.RNase yatangijwe mucyumba cyo gukoreramo cyangwa nta gants imwe ikoreshwa, masike, nibindi byambarwa.
Igitekerezo: Gukuramo igeragezwa rya molekile ya RNA bikorwa neza mubyumba bitandukanye bya RNA, kandi imbonerahamwe yubushakashatsi isukurwa mbere yubushakashatsi.Wambare uturindantoki hamwe na mask mugihe cyo kugerageza kugirango wirinde kwangirika kwa RNA guterwa na RNase.
4.Ragent yandujwe na RNase mugihe cyo kuyikoresha.
Icyifuzo: Simbuza Virus RNA nshya yo kwigunga kubushakashatsi bujyanye.
5.Indwara ya RNase yanduye ya centrifuge, inama za pipeti, nibindi. Icyifuzo: Menya neza ko imiyoboro ya centrifuge, inama za pipeti, hamwe na pipeti byose ari RNase-Free.
Molekile ya RNA yatunganijwe yagize ingaruka kubushakashatsi bwo hasi
Molekile ya RNA yatunganijwe ninkingi yo kweza izagira ingaruka kubigeragezo byo hepfo niba hari ion nyinshi cyangwa umunyu mwinshi, nka: transcription transcription, Blot y'Amajyaruguru, nibindi .。
1.Hariho ion z'umunyu zisigaye muri molekile ya RNA yatowe.
Icyifuzo: Menya neza ko ingano yukuri ya etanol ya anhydrous yongerewe kuri Buffer viRW2, hanyuma ukarabe inkingi yo kweza kabiri ukurikije umuvuduko ukwiye wa centrifugation kumabwiriza yimikorere ； Niba hakiri ioni yumunyu usigaye, urashobora kongeramo Buffer viRW2 kumurongo wogusukura, hanyuma ukayirekera mubushyuhe bwicyumba cya 5min.Noneho kora centrifugation kugirango ukureho umunyu ion wanduye kurwego runini
2.Hariho Ethanol isigaye muri molekile ya RNA yatowe
Igitekerezo: umaze kwemeza ko inkingi zo kwezwa zogejwe na Buffer viRW2, kora centrifugation yubusa-yubusa ukurikije umuvuduko wa centrifugal kumabwiriza yimikorere.Niba hasigaye etanol isigaye, irashobora gusigara muminota 5 mubushyuhe bwicyumba nyuma ya centrifugation yubusa kugirango ikureho Ethanol isigaye kurwego runini.
Imfashanyigisho:
Igitabo gikubiyemo amabwiriza ya virusi ya RNA
