Foregene ADN Yerekana Sisitemu Y Yongeyeho (Kudakuramo)
Ibisobanuro:
ForegeneSisitemu yo kumenyekanisha ADN Y Plus ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 20 Y chromosome yibanze, 15 ikunzwe, 6 isanzwe hamwe na 3 Y indel icyarimwe.
Ibisobanuro
ForegeneSisitemu yo kumenyekanisha ADN Y Plus ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 20 Y chromosome yibanze, 15 ikunzwe, 6 isanzwe hamwe na 3 Y indel icyarimwe.
Ibirimo
Gutegura sisitemu yo gutegura
UwitekaimyiteguroBya standard(gukuramo)sisitemu yo kongera imbaraga
| CIbigize | 25 μlSisitemu (μl) | Sisitemu ya 10μl (μl) | |
| Master mix | 4 Mix Master mixVII | 6.25 | 2.5 |
| 5 × Y Yongeyeho Primer ivanze | 5.0 | 2.0 | |
| Amazi yimana | 13.75 | 5.5 | |
| Blood ikizinga | Diameter 1.2mm | Diameter 1.2mm | |
| Tingano ya reaction | 25 μl | 10 μl | |
Isesengura ryamakuru
Umugereka wa 1:Ikarita Yandika Ikarita
Umugereka wa 2:Kwandika ADN isanzwe 9948ikarita
Ububiko bwa Reagent
1. Nyamuneka nyamuneka ubike munsi ya -20 ° C (Niba bidakoreshejwe by'agateganyo), nyuma yo kwakira ibikoresho bikonje mu rubura rwumye cyangwa paki ya gel.
2. Nyamuneka nyamuneka ubike ibice byabanjirije reaction 4 × premix VII kuri -20 ° C, nyuma yuko ibikoresho bimaze gukurwa kugirango bikoreshwe, nibindi bisigaye mbere yo kubyitwaramo bibikwa kuri 4 ° C kugirango wirinde gukonja no gukonja.Niba igipimo kimwe ari gito, birasabwa kubika kuri -20 ° C nyuma yo gutandukana.
3. Bika ibice nyuma yo kubyitwaramo kuri 4 ° C, irinde gukonjesha no gukonja inshuro nyinshi, kandi ntukore kuri reagent mbere yo kubyitwaramo kugirango wirinde kwanduza.
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