Amaraso RNA Igikoresho cyo kwigunga
Ibisobanuro:
Injangwe.RE-04011/04013
Kugirango RNA isukure mumaraso yose 104 Cell Amaraso yera yera ≤ 107
Gutandukanya vuba no kweza amaraso RNA muri selile yera.
-Ntabwo ukeneye guhangayikishwa no kwangirika kwa RNA.Ibikoresho byose ni RNase-Ubuntu
-Icyoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba
-Byihuta-ibikorwa birashobora kurangira muminota 20
-Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA
-Umutekano-nta reagent ikoreshwa
-Ubushobozi bunini bwo gutunganya-bigera kuri 200μl byintangarugero birashobora gutunganywa buri gihe.
-Ubuziranenge bwo hejuru - RNA isukuye ni nziza cyane, idafite poroteyine n’indi mwanda, kandi irashobora guhura nuburyo butandukanye bwo kugerageza.
Ibisobanuro
Igikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo neza isuku nini kandi yuzuye ya RNA yuzuye mumaraso yose arwanya antikagile.Igikoresho gitanga selile yumutuku lysate (Buffer RCL), ishobora kwihuta kandi neza ingirabuzimafatizo zitukura kandi ikagumana selile yera.Colunm ikora neza ya ADN irashobora gutandukanya byoroshye lysates ndengakamere na adsorb no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe;Inkingi ya RNA yonyine irashobora guhuza neza RNA, kandi hamwe na formula idasanzwe, irashobora gutunganya umubare munini wintangarugero icyarimwe.
Sisitemu yose RNase-Free ituma RNA yakuweho idasenyuka;Sisitemu yo gukaraba ya Buffer RW1 na Buffer RW2 ituma RNA yabonetse idafite proteyine, ADN, ion hamwe n’umwanda w’ibinyabuzima.
Ibirimo
| Amaraso Yuzuye RNA Igikoresho cyo kwigunga | ||
| Ibigize ibikoresho | RE-04011 | RE-04013 |
| Inshuro 50 | Inshuro 200 | |
| Buffer RCL (10 ×) | 52.5 mL | 210mL |
| Buffer BRL1 * | 30mL | 120mL |
| Buffer BRL2 | 18mL | 66mL |
| Buffer RW1 * | 25mL | 100mL |
| Buffer RW2 | 24mL | 96mL |
| Ubusa ddH2 O | 10mL | 40mL |
| Inkingi ya RNA gusa | Amaseti 50 | Amaseti 200 |
| Inkingi ya ADN | Amaseti 50 | Amaseti 200 |
| imfashanyigisho | Kopi 1 | Kopi 1 |
Ibiranga ibyiza
-Ntabwo ukeneye guhangayikishwa no kwangirika kwa RNA.Ibikoresho byose ni RNase-Ubuntu.
-Icyoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba.
-Byihuta-ibikorwa birashobora kurangira muminota 20.
-Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
-Umutekano-nta reagent ikoreshwa.
-Ubushobozi bunini bwo gutunganya-bigera kuri 200μl byintangarugero birashobora gutunganywa buri gihe.
-Ubuziranenge bwo hejuru - RNA isukuye ni nziza cyane, idafite poroteyine n’indi mwanda, kandi irashobora guhura nuburyo butandukanye bwo kugerageza.
Ibipimo by'ibikoresho
Gusaba ibikoresho:
Irakwiriye gukuramo no kweza RNA yose mumaraso yinyamabere.
Urujya n'uruza rw'akazi
Imiterere yo kubika
Buffer RCL (10 ×) igomba kubikwa kuri 2-8 ℃;ibindi bice bigize ibikoresho birashobora kubikwa mubushyuhe bwicyumba (15-25 ℃) mugihe cyumye, kandi birashobora kubikwa amezi 12.Buffer BRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc-mercaptoethanol (bidashoboka).
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Witondere gushyira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka mbere yo gukoresha.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange neza mbere yo kubikoresha.
Imiyoboro yo gusesengura ibibazo
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Nta RNA ishobora gukururwa cyangwa umusaruro wa acide nucleic ni muke
Mubisanzwe hariho ibintu byinshi bigira ingaruka kumikorere yo gukira, nka: icyitegererezo cya RNA, uburyo bwo gukora, ingano ya elution, nibindi .。
Isesengura ry'impamvu zisanzwe ：
1.Kora ubwogero cyangwa ubushyuhe buke (4 ° C) centrifugation mugihe ukora.
Icyifuzo: Ubushyuhe bwicyumba (15-25 ° C) imikorere, ntuzigere woga urubura hamwe na centrifuge yubushyuhe buke.
2. Kubika icyitegererezo kidakwiye cyangwa ububiko bw'icyitegererezo igihe kirekire.
Icyifuzo: Bika ingero kuri -80 ° C cyangwa uhagarike muri azote yuzuye, kandi wirinde gukoresha inshuro nyinshi;gerageza gukoresha ingero zegeranijwe zivuye muri RNA.
3.Icyitegererezo kidahagije
Icyifuzo: Nyamuneka menya neza ko icyitegererezo nigisubizo cyakazi (Linear Acrylamide) byavanze neza kandi bigashyirwa muminota 10 mubushyuhe bwicyumba (15-25 ° C)
4.Umuhanga wongeyeho nabi
Icyifuzo: Menya neza ko ddH2O yubusa ya RNase yongewe hagati ya membrane yinkingi yo kweza
5.Ubunini budakwiye bwa anhydrous ethanol muri Buffer viRW2
Igitekerezo: Nyamuneka kurikiza amabwiriza, ongeramo ingano yukuri ya anhydrous ethanol kuri Buffer viRW2 hanyuma ubivange neza mbere yo gukoresha ibikoresho。
6.Ikoreshwa ry'icyitegererezo kidakwiye.
Igitekerezo: 200µl yicyitegererezo kuri 500μl ya Buffer viRL.Ingano yikitegererezo irenze izagabanya igipimo cyo gukuramo RNA.
7.Ijwi ridakwiriye cyangwa gusohora kutuzuye.
Igitekerezo: Ingano nziza yinkingi yo kweza ni 30-50μl;niba ingaruka zo gukuraho zidashimishije, birasabwa kongeramo ubushyuhe bwa RNase-Ubusa ddH2O hanyuma wongere umwanya ushira mubushyuhe bwicyumba, nka 5-10min
8.Inkingi yo kweza ifite ibisigisigi bya Ethanol nyuma yo koza muri Buffer viRW2.
Igitekerezo: Niba Ethanol ikomeje kuguma nyuma yo koza muri Buffer viRW2 hamwe na centrifugation yubusa ya 2min, inkingi yo kweza irashobora gusigara mubushyuhe bwicyumba cya 5min nyuma ya centrifugation yubusa kugirango ikureho Ethanol isigaye.
Kwangirika kwa molekile ya RNA isukuye
Ubwiza bwa RNA yatunganijwe bujyanye nibintu nko kubika icyitegererezo, kwanduza RNase, no gukora.
Isesengura ry'impamvu zisanzwe ：
1.Icyitegererezo cyegeranijwe nticyabitswe mugihe.
Igitekerezo: Niba icyitegererezo kidakoreshejwe mugihe cyo gukusanya, nyamuneka ubike kuri -80 ℃ cyangwa azote yuzuye.Kubikuramo molekile ya RNA, gerageza gukoresha ingero zegeranijwe igihe cyose bishoboka.
2.Ingero zegeranijwe zarakonje kandi zishonga inshuro nyinshi.
Igitekerezo: Irinde gukonjesha no gukonjesha inshuro nyinshi (bitarenze rimwe) mugihe cyo gukusanya no kubika, bitabaye ibyo umusaruro wa acide nucleique uzagabanuka.
3.RNase yatangijwe mucyumba cyo gukoreramo cyangwa nta gants imwe ikoreshwa, masike, nibindi byambarwa.
Igitekerezo: Gukuramo igeragezwa rya molekile ya RNA bikorwa neza mubyumba bitandukanye bya RNA, kandi imbonerahamwe yubushakashatsi isukurwa mbere yubushakashatsi.Wambare uturindantoki hamwe na mask mugihe cyo kugerageza kugirango wirinde kwangirika kwa RNA guterwa na RNase.
4.Ragent yandujwe na RNase mugihe cyo kuyikoresha.
Icyifuzo: Simbuza Virus RNA nshya yo kwigunga kubushakashatsi bujyanye.
5.Indwara ya RNase yanduye ya centrifuge, inama za pipeti, nibindi. Icyifuzo: Menya neza ko imiyoboro ya centrifuge, inama za pipeti, hamwe na pipeti byose ari RNase-Free.
Molekile ya RNA yatunganijwe yagize ingaruka kubushakashatsi bwo hasi
Molekile ya RNA yatunganijwe ninkingi yo kweza izagira ingaruka kubigeragezo byo hepfo niba hari ion nyinshi cyangwa umunyu mwinshi, nka: transcription transcription, Blot y'Amajyaruguru, nibindi .。
1.Hariho ion z'umunyu zisigaye muri molekile ya RNA yatowe.
Icyifuzo: Menya neza ko ingano yukuri ya etanol ya anhydrous yongerewe kuri Buffer viRW2, hanyuma ukarabe inkingi yo kweza kabiri ukurikije umuvuduko ukwiye wa centrifugation kumabwiriza yimikorere ； Niba hakiri ioni yumunyu usigaye, urashobora kongeramo Buffer viRW2 kumurongo wogusukura, hanyuma ukayirekera mubushyuhe bwicyumba cya 5min.Noneho kora centrifugation kugirango ukureho umunyu ion wanduye kurwego runini
2.Hariho Ethanol isigaye muri molekile ya RNA yatowe
Igitekerezo: umaze kwemeza ko inkingi zo kwezwa zogejwe na Buffer viRW2, kora centrifugation yubusa-yubusa ukurikije umuvuduko wa centrifugal kumabwiriza yimikorere.Niba hasigaye etanol isigaye, irashobora gusigara muminota 5 mubushyuhe bwicyumba nyuma ya centrifugation yubusa kugirango ikureho Ethanol isigaye kurwego runini.
Imfashanyigisho:
Igitabo gikubiyemo amabwiriza ya virusi ya RNA
