BCR ABL1 Amabara abiri Amashanyarazi abiri
Ibisobanuro:
Dukurikije ihame rya ADN yuzuzanya, iperereza rya ABL orange-umutuku na BCR icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Dukurikije ihame rya ADN yuzuzanya, iperereza rya ABL orange-umutuku na BCR icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| 5Ibizamini | 10Ibizamini | 20Ibizamini | |
| BCR ABL1Amabara abiri Amashanyarazi abiri | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane ubuziranenge bwa gene muntu ya BCR / ABL mu byitegererezo bya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
Ku maraso yo mu magufa cyangwa ku maraso ya peripheri, icyitegererezo gishya kidakwiye kubikwa kuri 4 ° C mu gihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo bigomba kubikwa kuri -20 ° C.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
