Foregene Sisitemu yo Kumenyekanisha ADN 30 AC (Urubanza)
Ibisobanuro:
Foregene Sisitemu yo kumenya ADN30A-C ikoresha amabara atandatu ya fluorescent labels tekinoroji kuriongeraho 29autosomalSTR loci, 1 Inzige ya Amel, 1 Y.indelinzige, na1 ubwoko bwabantu bwihariye Imibonano mpuzabitsina, 2imbuga zo kugenzura ubuziranenge bwimbere, zikoreshwa mugukomezaADNInyandikorugero yakuweho kandi isukuwe kuvaCibikoresho bya ase.
Ibisobanuro
Foregene Sisitemu yo kumenya ADN30A-C ikoresha amabara atandatu ya fluorescent labels tekinoroji kuriongeraho 29autosomalSTR loci, 1 Inzige ya Amel, 1 Y.indelinzige, na1 ubwoko bwabantu bwihariye Imibonano mpuzabitsina, 2imbuga zo kugenzura ubuziranenge bwimbere, zikoreshwa mugukomezaADNInyandikorugero yakuweho kandi isukuwe kuvaCibikoresho bya ase.
Ibirimo
Gutegura sisitemu yo gutegura
Imbonerahamwe 2: Gutegura ibice byo gukuramo bisanzwe hamwe na sisitemu yo kongera imbaraga
|
| CIbigize | 25ul sisitemu (µl) | Sisitemu ya 10μl (μl) |
| Master mix | 2.5 × Ijambo ryibanzeCⅡ | 10.0 | 4.0 |
| 5 x 30 AC Primer mix | 5.0 | 2.0 | |
| Inyandikorugero ya ADN | 2.5-10 | 1—4 | |
| Amazi yimana | Gukora kugeza kuri reaction ya 25μl | Gukora kugeza kuri reaction ya10 μl |
Isesengura ryamakuru
Umugereka wa 1:Ikarita Yandika Ikarita
Umugereka wa 2:Kwandika ADN isanzwe 9948ikarita
Ububiko bwa Reagent
1. Nyamuneka nyamuneka ubike munsi ya -20 ° C ,, nyuma yo kwakira ibikoresho bikonje mu rubura rwumye cyangwa paki ya pisine.
2. Nyamuneka nyamuneka ubike ibice byabanjirije reaction 2.5 × premix CII kuri -20 ° C nyuma yuko ibikoresho bisohotse kugirango bikoreshwe ,, hanyuma reagent zisigaye zibikwa kuri 4 ° C kugirango wirinde gukonjeshwa no gukonja.Niba igipimo kimwe ari gito, birasabwa kubika kuri -20 ° C nyuma yo gutandukana.
3. Bika ibice nyuma yo kubyitwaramo kuri 4 ° C, irinde gukonjesha no gukonja inshuro nyinshi, kandi ntukore kuri reagent mbere yo kubyitwaramo kugirango wirinde kwanduza.
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