Foregene ADN Sisitemu Yerekana 38Y-C (Urubanza)
Ibisobanuro:
Foregene Sisitemu yo kumenya ADN 38Y-C ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 38 Y chromosome STR loci, 3 Y-Indelnabibiri byimbere byimbere kugenzura icyarimwe icyarimwekuri amplification Urugero rwakuwe mubishusho bya ADN bisukuye.
Ibisobanuro
Foregene Sisitemu yo kumenya ADN 38Y-C ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 38 Y chromosome STR loci, 3 Y-Indelnabibiri byimbere byimbere kugenzura icyarimwe icyarimwekuri amplification Urugero rwakuwe mubishusho bya ADN bisukuye.
Ibirimo
Gutegura sisitemu yo gutegura
Uwitekaimyiteguron of Bisanzwegukuramona sisitemu yo kongera imbaraga
| Master mix | CIbigize | Sisitemu ya 10μl (μl) |
| 2.5 × PCR Master Mix C I. | 4.0 | |
| 5 x 38Y-C Ivanga rya Primer | 2.0 | |
| Inyandikorugero ya ADN | 1- 4 | |
| Amazi yimana | Uzuza kugeza kuri reaction ya10 μl |
Isesengura ryamakuru
Umugereka wa 1:Ikarita Yandika Ikarita
Umugereka wa 2:Kwandika ADN isanzwe 9948ikarita
Ububiko bwa Reagent
1. Nyamuneka ubibike munsi ya -20 ° C (niba bidakoreshejwe by'agateganyo), nyuma yo kwakira ibikoresho bikonje mu rubura rwumye cyangwa paki ya gel.
2. Nyamuneka sotre ibice byabanjirije reaction PCR ivanze ni kuri -20 ° C, nyuma yigikoresho gisohotse kugirango gikoreshwe naho reagent zisigaye zibikwa kuri 4 ° C kugirango wirinde gukonja no gukonja.Niba igipimo kimwe ari gito, birasabwa kubika kuri -20 ° C nyuma yo gutandukana.
3. Bika ibice nyuma yo kubyitwaramo kuri 4 ° C, irinde gukonjesha no gukonja inshuro nyinshi, kandi ntukore kuri reagent mbere yo kubyitwaramo kugirango wirinde kwanduza.
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