1. Nyamuneka nyamuneka ubike munsi ya -20 ° C ,, nyuma yo kwakira ibikoresho bikonje mu rubura rwumye cyangwa paki ya pisine.

2. Nyamuneka nyamuneka ubike ibice byabanjirije reaction 4 × premix VII kuri -20 ° C, nyuma yigikoresho cyakuweho kugirango ukoreshe, hanyuma ubike izindi reagent zisigaye mbere ya reaction kuri 4 ° C kugirango wirinde gukonja no gukonja.Niba igipimo kimwe ari gito, birasabwa kubika kuri -20 ° C nyuma yo gutandukana,

3. Bika ibice nyuma yo kubyitwaramo kuri 4 ° C, irinde gukonjesha no gukonja inshuro nyinshi, kandi ntukore kuri reagent mbere yo kubyitwaramo kugirango wirinde kwanduza.