Foregene Sisitemu yo Kumenyekanisha ADN 20A (Gukuramo ADN kubuntu)
Ibisobanuro:
Sisitemu yo kumenya ADN ya Foregene 20A ikoresha tekinoroji yamabara atanu ya fluorescent kugirango yongere icyarimwe icyarimwe 19 STR na 1 yi mibonano mpuzabitsina mu muyoboro umwe.Mugihe gikubiyemo 13 CODIS yibanze, 20 yibanze byujuje ibisabwa na minisiteri yumutekano rusange kubikoresho bya autosomal STR.Numuntu wiburyo bwububiko bwububiko bwa ADN no kumenyekanisha ubuvandimwe.
Ibisobanuro
Sisitemu yo kumenya ADN ya Foregene 20A ikoresha tekinoroji yamabara atanu ya fluorescent kugirango yongere icyarimwe icyarimwe 19 STR na 1 yi mibonano mpuzabitsina mu muyoboro umwe.Mugihe gikubiyemo 13 CODIS yibanze, 20 yibanze byujuje ibisabwa na minisiteri yumutekano rusange kubikoresho bya autosomal STR.Numuntu wiburyo bwububiko bwububiko bwa ADN no kumenyekanisha ubuvandimwe.
Ibirimo
| Ibikoresho bya Kit | Izina rya Componet | Umubumbe （μl / tube） | Umubare （tube） |
| PCR Imbere-reaction reagent | 2.5 × PCR reaction ya buffer Ⅲ | 1000 | 2 |
| 5 × 20A kuvanga primer | 500 | 2 | |
| Kugenzura ADN 9947A （1ng / μl） | 25 | 1 | |
| Amazi yimana | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Inyuma-reaction reagent | Urwego rwa Allelic | 40 | 1 |
| Uburemere bwa molekulari imbere imbereORG 500 | 150 | 2 | |
| Icyitonderwa | |||
Isesengura ryamakuru
1. Fungura software ya Genemapper.Mugihe ukoresheje iki gikoresho kunshuro yambere, ugomba gutumiza paneli, gushiraho bin, uburyo bwo gusesengura bujyanye, hamwe nubunini (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 330, 360, 390, 420, 450, 490, 500)
2. Kuzana amakuru ya electrophoreis, hitamo ibipimo bijyanye nisesengura nkibibaho, uburyo bwo gusesengura, nubunini bwubunini, hanyuma uhindure ubwoko bwicyitegererezo cyurwego kuri "Allelic Ladder" mumurongo "Ubwoko bw'icyitegererezo";tangira gusesengura amakuru.
Ububiko bwa Reagent
1. Nyuma yo kwakira igikoresho cyakonjeshejwe mu rubura rwumye cyangwa mu bikoresho bya pulasitike, niba bidakoreshejwe by'agateganyo, nyamuneka ubibike munsi ya -20 ° C igihe kirekire.
2. Igikoresho kimaze gukurwa no gukoreshwa, ibikoresho bigomba kubikwa kuri 4 ° C mbere yo kubyitwaramo kugirango wirinde gukonjeshwa no gukonja;Enzyme ya Taq igomba kubikwa kuri -20 ° C.
3. Nyuma yo kubyitwaramo, ibikoresho (harimo uburemere bwimbere bwimbere hamwe nintambwe) bigomba kubikwa muri "electrophoresis detection room" kuri 4 ° C, ukirinda gukonjesha no gukonjesha inshuro nyinshi, kandi wirinde guhura nibikoresho byabanjirije reaction kugirango wirinde kwanduza.
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