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Foregene Sisitemu yo Kumenyekanisha ADN 27Y

Foregene ADN Sisitemu Yerekana 27Y Ishusho Yerekanwe
  • Foregene Sisitemu yo Kumenyekanisha ADN 27Y
  • Foregene Sisitemu yo Kumenyekanisha ADN 27Y
  • Foregene Sisitemu yo Kumenyekanisha ADN 27Y

Ibisobanuro:

Foregene Sisitemu yo Kumenyekanisha ADN 27Y ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 27 Y-STR icyarimwe.Harimo: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYF387S1, DYS393, DYS389, DYS3904 481, DYS385, DYS449.

Igikoresho gifite ubushobozi bwiza bwo kurwanya-kubuza, kandi gishobora kwongerera mu buryo butaziguye impapuro ziyungurura, ikarita ya FTA, ipamba, ipamba, ikarita y'amacandwe, umunwa wo mu kanwa, kandi irashobora no gukoreshwa mu gukuramo no kweza inyandikorugero za ADN mu bikoresho.

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Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Foregene Sisitemu yo Kumenyekanisha ADN 27Y ikoresha tekinoroji yamabara atandatu ya fluorescent kugirango yongere 27 Y-STR icyarimwe.Harimo: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYF387S1, DYS393, DYS389, DYS3904 481, DYS385, DYS449.

Igikoresho gifite ubushobozi bwiza bwo kurwanya-kubuza, kandi gishobora kwongerera mu buryo butaziguye impapuro ziyungurura, ikarita ya FTA, ipamba, ipamba, ikarita y'amacandwe, umunwa wo mu kanwa, kandi irashobora no gukoreshwa mu gukuramo no kweza inyandikorugero za ADN mu bikoresho.

Ibirimo

Ibikoresho byo kwipimisha

Izina ryibigize

Umubumbe (μl / tube)

Umubare (tube)

PCR mbere yogukora ibikoresho

 2 × PCR Igikorwa cyo Kuvanga Master mix

1250

2
5 x 27Y Ivanga rya Primer

500

2
Imyandikire ya ADN 9948

20

2
Amazi yimana

1700

2

Inyuma ya PCR Igikoresho

 27Y allele ivanze Urwego

20

2
Ibiro bya orange uburemere bwimbere ORG500

150

2

Isesengura ryamakuru

1. Fungura software ya ID Mapper.Mugihe ukoresheje iki gikoresho kunshuro yambere, ugomba gutumiza panele, bin yashizweho, uburyo bwo gusesengura bujyanye, hamwe nubunini (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 330, 360, 390, 420, 450, 490, 500).

2. Kuzana amakuru ya electrophoreis, hitamo ibipimo bijyanye nisesengura nkibibaho, uburyo bwo gusesengura, nubunini bwubunini, hanyuma uhindure ubwoko bwicyitegererezo cyurwego kuri "Allelic Ladder" mumurongo "Ubwoko bw'icyitegererezo";tangira gusesengura amakuru.

Ububiko bwa Reagent

1. Nyamuneka ubibike munsi ya -20 ° C (niba bidakoreshejwe by'agateganyo), nyuma yo kwakira ibikoresho bikonje mu rubura rwumye cyangwa paki ya gel.

2. Nyamuneka nyamuneka ubike ibikoresho kuri 4 ° C mbere yo kubyitwaramo kugirango wirinde gukonjesha no gukonja inshuro nyinshi, nyuma yo gukuramo ibikoresho,;enzyme igomba kubikwa kuri - 20 ° C.

2. Nyuma yo kubyitwaramo, ibikoresho (harimo uburemere bwimiterere yimbere hamwe nintambwe) bigomba kubikwa muri "electrophoresis detection room" kuri 4 ° C, ukirinda gukonjesha no gukonjesha inshuro nyinshi, kandi wirinde guhura nibikoresho byabanjirije reaction kugirango wirinde kwanduza.

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