Foregene Sisitemu yo Kumenyekanisha ADN 25A-C (Urubanza)
Ibisobanuro:
Sisitemu yo kumenya ADN ya Foregene 25A-C ikoresha tekinoroji yamabara atandatu ya fluorescence kugirango yongereze 23 autosomal STR loci, imwe Amel loci, Yindel loci imwe na loci ebyiri zo kugenzura ubuziranenge bwimbere icyarimwe kugirango hongerwe inyandikorugero ya ADN yakuwe kandi isukuwe mubitegererezo.
Ibisobanuro
Sisitemu yo kumenya ADN ya Foregene 25A-C ikoresha tekinoroji yamabara atandatu ya fluorescence kugirango yongereze 23 autosomal STR loci, imwe Amel loci, Yindel loci imwe na loci ebyiri zo kugenzura ubuziranenge bwimbere icyarimwe kugirango hongerwe inyandikorugero ya ADN yakuwe kandi isukuwe mubitegererezo.
Ibirimo
Gutegura sisitemu yo gutegura
Gutegura Ibigize bisanzwe byo gukuramo no kongera imbaraga
| Ibigize | Sisitemu ya 10μl (μl) | |
| Master mix | 2.5 × PCR Ikibanza C Ⅰ | 4.0 |
| 10 × 25A-Kuvanga | 1.0 | |
| Inyandikorugero ya ADN | 1-5 | |
| Amazi yimana | Gukora kugeza kuri reaction ya 10μl |
Isesengura ryamakuru
Umugereka wa 1:Urwego genotyping ya allele
Umugereka wa 2:Kwandika ADN isanzwe 9948ikarita
Ububiko bwa Reagent
1. Nyuma yo kwakira ibikoresho, nyamuneka ubike munsi -20 ℃ niba udakoresheje umwanya uriho.
2. Nyuma yo gukuramo ibikoresho no gukoreshwa, igisubizo kibanziriza reaction PCR kibanziriza igisubizo kibikwa kuri -20 and, naho reagent zisigaye zabanjirije kubikwa kuri 4 ℃ kugirango wirinde gukonjeshwa.Niba igipimo kimwe ari gito, birasabwa kubika kuri -20 ° C nyuma yo gupakira.
3. Nyuma yo kubyitwaramo, ibice bibikwa kuri 4 ℃ kugirango wirinde gukonjeshwa.Ntugakore kuri reagent mbere yo kwitwara kugirango wirinde umwanda.
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