RNAlater (Kubijyanye na RNA) Igisubizo cya RNAlater
Ibisobanuro:
Gukosora byihuse inyama zintangarugero zafashwe vuba, kubuza ibikorwa bya RNase, kurinda RNA kwangirika.
-Reagent ikoreshwa muburyo butaziguye, intambwe imwe irahari, kandi RNA ihita itekana kandi ikarindwa.
-Gukorera mubushyuhe bwicyumba, byoroshye, umutekano kandi ntabwo ari uburozi.
-Kugira ngo ukureho ibibazo bya barafu yumye cyangwa firigo yo hasi yubushyuhe bwo kubika tissue.
-Tissue irashobora kubikwa igihe kirekire nta ngaruka zo kwangirika kwa RNA, ikemeza amakuru yizewe yerekana imiterere yamakuru.
Ibisobanuro
RNAlater ni amazi adafite ubumara bwinyamanswa zo kubika reagent.Irashobora kwinjira vuba mu ngirabuzimafatizo kandi ikarinda RNA ingirabuzimafatizo idakonjeshejwe no kwangirika mu guhagarika neza ibikorwa bya RNase, ku buryo nyuma yo kubona icyitegererezo cy'inyama, bidakenewe gutunganywa ako kanya, kandi ntabwo ari ngombwa guhagarika icyitegererezo muri azote yuzuye., bityobiroroshye cyane kubikorwa byubushakashatsi.Gukoresha tissue yo gukingira RNA birashobora kwirinda ikibazo cyo gukoresha azote ya azote cyangwa firigo ikabije yubushyuhe bukabije, kandi kubika ibyiciro bitandukanye byintangarugero mubisubizo byuburinzi birashobora guhita bihagarara kandi bigakosora impinduka zikurikirana zerekana imvugo ya RNA, ishobora kugabanya ikosa riri hagati yitsinda ryubushakashatsi.
RNAlater irashobora gukoreshwa cyane muburorero butandukanye bwintangangabo, harimo ubwonko, umutima, impyiko, impyiko, umwijima, nibihaha.Nyuma yuko imyenda mishya idakonje yibijwe muri Buffer RNAlater ku kigereranyo cya 1:10, ingero zirashobora kubikwa mubushyuhe bwicyumba icyumweru 1, 37 ° C kumunsi 1, 4 ° C byibuze ukwezi;imyenda irashobora kwibizwa kuri 4 ° C kuri -20 ° C cyangwasyatanyaguwe kuri -80 ℃ igihe kirekire.
Ibisobanuro
Ml 50, ml 100, 100 * 4 ml
Ibigize ibikoresho
| RNAnyumaKubijyanye na RNA | |||
| Injangwe. | RL-01011 | RL-01012 | RL-01013 |
| Buffer RNAnyuma | 50ml | 100ml | 100ml × 4 |
| Igitabo gikubiyemo amabwiriza | 1 igice | 1 igice | 1 igice |
Ibiranga ibyiza
-Reagent ikoreshwa muburyo butaziguye, intambwe imwe irahari, kandi RNA ihita itekana kandi ikarindwa.
-Gukorera mubushyuhe bwicyumba, byoroshye, umutekano kandi ntabwo ari uburozi.
-Kugira ngo ukureho ibibazo bya barafu yumye cyangwa firigo yo hasi yubushyuhe bwo kubika tissue.
-Tissue irashobora kubikwa igihe kirekire nta ngaruka zo kwangirika kwa RNA, ikemeza amakuru yizewe yerekana imiterere yamakuru.
Ibipimo by'ibikoresho
RNAnyuma Gusaba
Irakoreshwa muguhita ibika inyamanswa zegeranijwe vuba kandi igahita ituza RNA murugero rwinyama kugirango ibungabunge imiterere ya gene.
Imiterere yo kubika
Irashobora kubikwa neza mugihe kirenze umwaka 1 mubushyuhe bwicyumba (15-25 ℃).
