PCR Intwari
Ibisobanuro:
◮Ubudahemuka bwo hejuru: 6ibihe bya enzyme isanzwe ya Taq;
◮Byihuse aumuvuduko wa mplification:umuvuduko wa amplification ni 5-10sec / kb, bikubye inshuro 6-12 iy'umusemburo wa Taq usanzwe;
◮Inyandikorugero nyinshi: irashobora kwagura neza inyandikorugero zitandukanye za ADN zifite ibintu byinshi bya GC kandi bigoye kubyongera;
◮HejuruGukora neza: umubare wikizunguruka cyikigereranyo kiri munsi yumusemburo wa Taq usanzwe;
◮Stronger Kwihanganira ibidukikije: yashyizwe kuri 37 ° C icyumweru, ikomeza ibirenze90%ibikorwa;
◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.
Ibisobanuro
Iki gicuruzwa kirimo Foregene idasanzwe idasanzwe ya Taq —— Foregene Taq Intwari, DNTPs nziza, MgCl2, reaction ya reaction, PCR yongera reaction, optimizer na stabilisateur.2 × Intwari ya PCRTMSisitemu yo kuvanga ifite kwihanganira cyane inhibitori za PCR kuruta sisitemu isanzwe ya PCR ivanze, kandi irashobora guhangana byoroshye na PCR ikwirakwizwa rya templates zitandukanye.Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq Intwari ituma reaction ya PCR igira ubushobozi bwo kongera amplification, umwihariko hamwe na sensitivite.
Ibisobanuro
Urukurikirane rw'ibikoresho
PH-01013 ： 2 × Intwari ya PCRTMKuvanga 1.7ml × 3
PH-01014 ： 2 × Intwari ya PCRTMKuvanga 1.7ml × 6
PH-01015 ： 2 × Intwari ya PCRTMKuvanga 1.7ml × 24
Ibigize ibikoresho
2 × Intwari ya PCRTMKuvanga harimo ： 0.1U Taq Intwari / μl, 400μM dNTPs, 20mM Tris-HCl (pH8.8), 100mM KCl, 5mM MgCl2, izindi stabilisateur hamwe nabazamura.
Ibiranga ibyiza
-Ubudahemuka bwo hejuru:6ibihe bya enzyme isanzwe ya Taq;
- Byihuse aumuvuduko wa mplification:umuvuduko wa amplification ni 5-10sec / kb, bikubye inshuro 6-12 iy'umusemburo wa Taq usanzwe;
-Inyandikorugero nyinshi: irashobora kwagura neza inyandikorugero zitandukanye za ADN zifite ibintu byinshi bya GC kandi bigoye kubyongera;
- HejuruGukora neza: umubare wikizunguruka cyikigereranyo kiri munsi yumusemburo wa Taq usanzwe;
-Stronger Kwihanganira ibidukikije: yashyizwe kuri 37 ° C icyumweru, ikomeza ibirenze90%ibikorwa;
-Ifite ibikorwa 5 '→ 3' ibikorwa bya ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.
Porogaramu
- PCR yongerewe ibice bya ADN
- Ikimenyetso cya ADN
- Urutonde rwa ADN
- PCR wongeyeho umurizo
Ububiko nubuzima bwiza
Irashobora kubikwa igihe kirekire kuri -20 ° C;gukoreshwa kenshi, irashobora kubikwa kuri 4 ° C mugihe gito.
