Amaraso ADN Midi Kit (1-5ml) Gutandukanya Amaraso Midi Ibikoresho bya ADN biva mumaraso
Ibisobanuro:
Sukura vuba ADN ya genomic yo mu rwego rwo hejuru mumaraso ya anticagulée (1-5ml).
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Icyitegererezo cyamaraso kirimo umubare munini wuturemangingo twamaraso atukura hamwe na selile ya platel, bigatuma gutunganya amaraso bigorana.Nyamara, ADN yamaraso yakuwe muburyo rusange ntabwo iri hejuru mubyera kandi ibirimo ni bike cyane.Iki gikoresho gikoresha uburyo bushya bwa Foregene Protease Plus hamwe na sisitemu yihariye ya BL1 na BL2, ishobora gusya rwose amaraso ya anticagulée kandicoagulationingero mugihe gito, bityo wirinde kwangirika kwa ADN ku rugero runini no kubona ADN nyinshi.Sisitemu yihuse yo gutunganya amaraso hamwe no gukora spin inkingi yoroshye byoroshya cyane gukuramo genome yamaraso, kugirango ADN genomique yo mu rwego rwo hejuru, ifite isuku nyinshi iboneke muminota 40.
ADN yonyine ya silika gel membrane ikoreshwa muri spin inkingi yiki gikoresho ni ibintu bishya bidasanzwe byikigo, bikora neza kandi byumwihariko byamamaza ADN, kandi bishobora gukuraho RNA, poroteyine zanduye, ion nibindi bintu kama kama mungirabuzimafatizo ku rugero runini.Ibice bya ADN byabonetse ni binini, bifite isuku nyinshi, bihamye kandi byizewe, kandi ubushobozi bwo gutwara inkingi ya spin ni 80 μg ADN.ADN yabonetse irashobora gukoreshwa mubushakashatsi bwibinyabuzima bwa molekuline nko kubuza igogorwa, PCR, kuvanga amajyepfo, kubaka amasomero nibindi.Igikoresho kirashobora gutunganya amaraso agera kuri 5ml icyarimwe kugirango ibone urugero ruhagije rwa ADN genomic.
Ibisobanuro
50 Imyiteguro, 100 Itegura, 250 Itegura
Ibigize ibikoresho
| Buffer BL1 |
| Buffer BL2 * |
| Buffer DC |
| Buffer PW * |
| Buffer WB1 |
| Buffer EB |
| Foregene Protease Yongeyeho |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kuruta protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 40.
-Ibyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, kandi ntihakenewe 4°C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Birakwiriye gukuramo no kweza ADN genomic mumaraso mashya cyangwa akonje.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25℃);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8℃.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease Plus igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kirekire (amezi 3), ibikorwa byayo nibituza bizaba byiza iyo bibitswe kuri 4 ° C, bityo rero birasabwa kubibika kuri 4 ° C, wibuke kutabika kuri -20℃.
