RT-qPCR Byoroshyeᵀᴹ (Intambwe imwe) -Taqman
Ibisobanuro:
- Umwe-intambwe yintambwe ituma transcript ihinduka na PCR ikorerwa mumiyoboro imwe, gusa ukeneye kongeramo inyandikorugero RNA, primers yihariye ya PCR na ddH yubusa ya RNase.2O.
- Isesengura nyaryo ryuzuye rya virusi ya RNA cyangwa RNA irashobora gukorwa vuba kandi neza.
- Igikoresho gikoresha uburyo bwihariye bwa Foregene revers transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yo gukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
- Sisitemu yogukora neza ituma reaction igira ibyiyumvo byo hejuru byo gutahura, gukomera kwumuriro, no kwihanganira neza.
- RT-qPCR BiroroshyeTM.
Ibisobanuro
Foregene Intambwe imwe RT-PCR Ibicuruzwa byuruhererekane byoroshye kumenya reaction ihuriweho kuva RNA ikagera kuri ADN igizwe na ADN ebyiri, ni ukuvuga ko kwandukura no guhinduranya PCR byarangiye muri rezo imwe ya centrifuge hamwe na sisitemu imwe ya reaction, bityo intambwe yubushakashatsi yoroshye, gahunda yubushakashatsi ikorwa neza, kandi imikorere yubushakashatsi ikanozwa.
RT-qPCR BiroroshyeTM (ImweIntambwe) -Taqmanukoresheje Foregene HotStar Taq ADN Polymerase, sisitemu nziza ya Taqman qPCR irashobora kwihuta kandi byumwihariko gukora Real Time RT-qPCR igereranya ryerekana ibimenyetso bya RNA.
Ibisobanuro
100 Rxns (sisitemu ya 20μl)
500 Rxns (sisitemu ya 20μl)
Ibigize ibikoresho
| RT-qPCR EasyTM (Intambwe imwe) -Taqman | ||
| Ibigize ibikoresho | RT-02131 | RT-02132 |
| 100Rxns (sisitemu ya 20μl) | 500 Rxns (sisitemu ya 20μl) | |
| 2 × RT-qPCR BiroroshyeTMKuvanga-Taqman | 1ml | 1.7ml × 3 |
| 20 × Irangi ryerekana irangi | 100μl | 0.5ml |
| RNase Yubusa ddH2O | 1.7ml | 1.7ml × 3 |
| Amabwiriza yo Gukoresha | 1 | 1 |
Ibiranga ibyiza
- Igikoresho kimwe cyintambwe ituma transcription yisubiramo na PCR ikorerwa mumiyoboro imwe, gusa ukeneye kongeramo inyandikorugero RNA, primers yihariye ya PCR na ddH yubusa ya RNase.2O.
- Isesengura nyaryo ryuzuye rya virusi ya RNA cyangwa RNA irashobora gukorwa vuba kandi neza.
- Igikoresho gikoresha uburyo bwihariye bwa Foregene revers transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yo gukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
- Sisitemu yogukora neza ituma reaction igira ibyiyumvo byo hejuru byo gutahura, gukomera kwumuriro, no kwihanganira neza.
- RT-qPCR BiroroshyeTM.
Ibipimo by'ibikoresho
Porogaramu
- Iki gikoresho gikoreshwa mugushakisha byihuse kandi neza ibimenyetso bya RNAs nka virusi ya RNA.
- Birakwiriye cyane cyane gutahura byihuse inyandikorugero za RNA zirimo GC nyinshi cyangwa imiterere ya kabiri igoye.
Urupapuro rw'akazi
Imiterere yo kubika
1.Ubwikoreziimiterere
Inzira yose yubushyuhe bwo hasi yubusanduku bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ℃.
2.Ububiko imiterere
Igikoresho kibitswe kuri -20 ℃.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri-20 ℃ ako kanya nyuma yo kwakirwa.Niba uburyo bwo kubika bukwiye, ibicuruzwantizitesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
