ByihuseEᵀᴹᵀᴹ Igihe nyacyo PCR Kit-Taqman
Ibisobanuro:
◮Sisitemu idasanzwe ya PCR yogukora ikora 2 × ByihuseTMPCR nyayo ivanze-Taqman irahuza cyane.
◮Gushyushya-gutangira Foregene HS Taq Polymerase ifite ubushobozi bwo kongera imbaraga, ibyiyumvo byiyongera, hamwe nuburyo bwihariye bwo kwongera.
◮2 × ByihuseTMReal PCR ivanze -Taqman ikoresha sisitemu idasanzwe yatunganijwe na Foregene kugirango itezimbere ibyiyumvo byihariye hamwe nubushakashatsi bwihariye bwo gutahura iperereza, bishobora gukoreshwa muri genotyping no gukoporora umubare ugereranya no kubona ibisubizo nyabyo.
◮Ibicuruzwa bizana irangi ryimbere rya ROX, rishobora gukoreshwa mugukuraho ibimenyetso byerekana ibimenyetso hamwe nikosa ryibimenyetso hagati yamariba, byorohereza abakiriya gukoresha muburyo butandukanye bwibikoresho bya PCR.
Ibisobanuro
2 × ByihuseTMPCR nyayo ivanze-Taqman yatanzwe na QuickEasyTMIgihe nyacyo PCR Kit-Taqman kit nigisekuru gishya cya master mix sisitemu yagenewe Real Time PCR amplification reaction ikoresheje fluorescent probe yihariye.Intangiriro yacyo ya Foregene HS Taq ADN Polymerase ishingiye ku guhindura Antibody, ikoreshwa ifatanije na Foregene kurushaho kunoza sisitemu ya PCR, ifite umwihariko wo gukora no kongera imbaraga.Ugereranije na PCR isanzwe ivanze, ifite imbaraga zo kongera imbaraga hamwe nigipimo cyo kudahuza.Irashobora gukoreshwa muri fluorescence yuzuye PCR reaction kugirango igabanye amplification idasanzwe no kunoza ukuri kwa PCR.Irashobora kunoza cyane ibicuruzwa byihariye no kubyumva neza.Muri icyo gihe, ROX yatanzwe nk'irangi ry'imbere.
ByihuseTMIgihe nyacyo PCR Kit-Taqman kit ifite intera nini yo gukoreshwa ku mubare w’icyitegererezo, kandi irashobora kubona umurongo mwiza ufatika mu turere dutandukanye, kandi irashobora kubara neza no kumenya genes zigenewe hamwe no gusubiramo neza kandi ufite ikizere kinini.
Ibisobanuro
200 Imyiteguro,500 Imyiteguro,1000 Imyiteguro,2000 Imyiteguro
Ibigize ibikoresho
| ByihuseTMIgihe nyacyo PCR Kit-Taqman(20μlSisitemu) | ||||
| Ibirimo | QP-01121 | QP-01122 | QP-01123 | QP-01124 |
| 200 Imyiteguro | 500 Imyiteguro | 1000 Imyiteguro | 2000 Imyiteguro | |
| 2 ×ByihuseBiroroshyeTM PCR nyayo Kuvanga-Taqman | 1 mL×2 | 1.7 mL×3 | 1.7 mL×6 | 1.7 mL×12 |
| 20×Irangi ryerekana irangi | 200μL | 0.5 mL | 1 mL | 1 mL×2 |
| DNase Yubusa ddH2O | 1.7 mL | 1.7 mL×2 | 10 mL | 20 mL |
| IFU | 1igice | 1igice | 1igice | 1igice |
Ibiranga ibyiza
System Sisitemu idasanzwe ya optimizasiyo ya PCR ikora 2 × ByihuseTMPCR nyayo ivanze-Taqman irahuza cyane.
■ Gushyushya-gutangira Foregene HS Taq Polymerase ifite ubushobozi bwo kongera amplification, sensibilité yo hejuru, hamwe na amplification yihariye.
■ 2× ByihuseTM Real PCR ivanze -Taqman ikoresha sisitemu idasanzwe yatunganijwe na Foregene kugirango itezimbere ibyiyumvo byihariye hamwe nubushakashatsi bwihariye bwo gutahura iperereza, bishobora gukoreshwa muri genotyping no gukoporora umubare ugereranya no kubona ibisubizo nyabyo.
■ Iki gicuruzwa kizana irangi ryimbere rya ROX, rishobora gukoreshwa mugukuraho ibimenyetso byerekana ibimenyetso hamwe nikosa ryibimenyetso hagati yamariba, byorohereza abakiriya gukoresha muburyo butandukanye bwibikoresho bya PCR.
Porogaramu
■ Fluorescence ingano ya PCR yo gusesengura ingano;
■ C.Kwiyongera kwa PCR;
■ Irashobora gukoreshwa mugushakisha allele.
Ububiko hamwe nubuzima bwa Shelf
Igikoresho kigomba kubikwa kuri -20 ° C mu mwijima;niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ° C mugihe gito (koresha muminsi 10).
Igihe nyacyo PCR primer igishushanyo mbonera
Imbere Primer na Reverse Primer
Kubihe Byukuri PCR, igishushanyo mbonera ni ngombwa cyane.Ibanze bifitanye isano nuburyo bwihariye bwo kongera PCR, kandi birashobora gushushanywa hifashishijwe amahame akurikira:
Uburebure bwa primer: 18-30bp.
Ibirimo GC: 40-60%.
Agaciro ka Tm: Porogaramu ishushanya, nka Primer 5, irashobora gutanga Tm agaciro ka primer.Indangagaciro za Tm zo hejuru no kumanuka primers zigomba kuba hafi bishoboka.Inzira yo kubara ya Tm irashobora kandi gukoreshwa: Tm = 4 ° C (G + C) + 2 ° C (A + T).Iyo ukora PCR, ubushyuhe buri munsi ya primer Tm agaciro ka 5 ° C muri rusange byatoranijwe nkubushyuhe bwa annealing (kwiyongera gukwiranye nubushyuhe bwa annealing birashobora kongera umwihariko wa reaction ya PCR).
Ibicuruzwa nibicuruzwa bya PCR:
Gushushanya primer PCR yongerera ibicuruzwa uburebure nibyiza 100-150bp.
Igishushanyo mbonera mugice cya kabiri cyubatswe cyicyitegererezo kigomba kwirindwa bishoboka.
Irinde gushiraho ibice 2 cyangwa byinshi byuzuzanya hagati ya 3 ′ impera yimbere na epfo na ruguru.
Primer 3 ′ itumanaho ntirishobora kuboneka hamwe na 3 yongeyeho G cyangwa C.
Primers ubwayo ntishobora kugira ibyuzuzanya, bitabaye ibyo imiterere yimisatsi izashyirwaho, bigira ingaruka kuri PCR.
ATCG igomba gukwirakwizwa uko bishoboka kwose murwego rwa primer, kandi 3 ′ base base igomba kwirindwa nka T.
Umugereka 1 ： Akagari kayobora RT-qPCR Ibikoresho byuzuza ibikoresho
1.Akagari ka Lysis
|
| |||
| Ibigize ibikoresho (24-neza lysis sisitemu / neza) | DRT-01011-A1 | DRT-01011-A2 | |
| 100 T. | 500 T. | ||
| IgiceI. | Buffer CL | 20 ml | 100 ml |
| Foregene Protease Yongeyeho II | 400 μl | 1 ml × 2 | |
| Buffer ST | 1 ml × 2 | 10 ml | |
| IgiceII | Gusiba ADN | 400 μl | 1 ml × 2 |
2.RT Kuvanga
|
| |
| Ibigize ibikoresho (20 μl sisitemu yo kwitwara) | DRT-01011-B1 |
| 200 T. | |
| 5 × Direct RT ivanze | 800 μl |
| Ubusa ddH2O | 1,7 ml × 2 |
|
| ||
| Ibigize ibikoresho (20 μl sisitemu yo kwitwara) | DRT-01011-C1 | DRT-01011-C2 |
| 200 T. | 1000 T. | |
| 2 × Direct qPCR ivanze-SYBR | 1 ml × 2 | 1,7 ml × 6 |
| 50 × ROX Yerekana irangi | 40 μl | 200 μl |
| Ubusa ddH2O | 1,7 ml | 10 ml |
-
Buccal Swab / Ikarita ya FTA ADN Yigunze Kit Genomic ...
-
Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye, smal ...
-
Foregene ADN Sisitemu Yerekana 38Y-C (Urubanza)
-
Zebra Ifi Direct PCR Kit-UNG Direct PCR Lysis ...
-
Intebe ya ADN Yigunze Kit ADN Gukuramo Purifica ...
-
MALT1 Amabara abiri Kumena Uretse iperereza
