Ubushinwa Zebra Ifi Direct PCR Kit-UNG Direct PCR Lysis Reagent (zebrafish) Ihingura nuwitanga |Foregene

Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent (zebrafish)

Ibisobanuro:

Gukoresha mu buryo butaziguye zebrafish lysate nk'icyitegererezo cyo kongera PCR, nta gukuramo ADN itandukanye, byihuse kandi byoroshye.Wongeyeho UNG yo kurwanya umwanda kugirango ikureho ibyiza.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, imirizo yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × PCR EasyTM ivanze (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2 × PCR EasyTM ivanze, ikongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo kikaba cyemeza umwihariko n'ukuri kwa amplification no gukumira ko hashobora kubaho ibizamini binini.Kwanduza ibicuruzwa bya PCR byabaye.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer FP
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga (UNG)
Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu yo kurwanya umwanda PCR 2 × PCR EasyTM Mix (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.