Imbuto y'ibihingwa Direct PCR Kit I / II (idafite ibikoresho byo gutoranya) Porotokole Igikoresho cya PCR Ibikoresho
Ibisobanuro:
◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)
◮Ibikoresho bike
◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50
◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR
Ibisobanuro
Iki gicuruzwa gikoresha sisitemu yihariye ya lysis buffer kugirango lyse imbuto yibimera.Lysate irashobora gukoreshwa nkicyitegererezo nta kweza.Nyuma yo kongeramo primers, PCR ivanze yiki gikoresho irashobora gukoreshwa muri amplification.
Ibisobanuro
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ibigize ibikoresho
| Imbuto y'ibihingwa Direct PCR Kit I. | |
| Igice cya I. | Buffer SP1 |
| Buffer SP2 | |
| 6 Buffer Yipakurura ADN | |
| Igice cya II | 2 × Imbuto PCR byoroshyeTMKuvanga |
| Imbuto y'ibihingwa Direct PCR Kit II | |
| Igice cya I. | Buffer SP1 |
| Buffer SP2 | |
| 6 Buffer Yipakurura ADN | |
| Igice cya II | 2 × Imbuto PCR byoroshyeTMKuvanga |
Ibiranga ibyiza
■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Biroroshye-icyitegererezo kirashobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
X 2X Kuvanga-kugabanya icyitegererezo cyo gupakira amakosa hamwe na sisitemu yo gutegura igihe
■ Byihuse-inyandikorugero irashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50
Kwinjira cyane-lysis reaction irashobora kurangizwa muri plaque 96-nziza PCR.
Ibipimo by'ibikoresho
Gushyira mu bikorwa: Kumenyekanisha ibimera byahinduwe, genotyping, nibindi.
Icyitegererezo: Tera imbuto cyangwa tissue zifite polysaccharide nkeya hamwe na polifenol.
Ingano yicyitegererezo: Imbuto imwe yose, 1-100mg yumubiri wibimera
Urutonde rwo kumenya: ibicuruzwa bya PCR ≤1kb
Urujya n'uruza rw'akazi
Amashanyarazi
-
Gutera ikibabi Direct PCR wongeyeho kit-UNG (udafite Sam ...
-
Amaraso SuperDirectᵀᴹ PCR Kit-EDTA Amaraso Direct P ...
-
Inyama zinyamanswa Direct PCR kit (nta Cyitegererezo ...
-
Amaraso SuperDirectᵀᴹ PCR Kit-Heparin Amaraso Direc ...
-
Ibiti byibiti bitaziguye PCR kit-UNG (nta Cyitegererezo ...
-
Imbeba Umurizo SuperDirect PCR Kit Direct PCR Lysis ...
