Ubushinwa Imbeba Umurizo SuperDirect PCR Kit Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping) Uwakoze nuwitanga |Foregene

Imbeba Umurizo SuperDirect PCR Kit Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)

Ibisobanuro:

Koresha mu buryo butaziguye imbeba umurizo lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer kugirango irekure vuba ADN ya genomic kuva umurizo wimbeba hamwe nintangarugero yugutwi kwimbeba ya PCR, bityo rero irakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri lysis buffer kirarangiyemu minota 10-30kuriubushyuhe bw'icyumba (20-25° C.).Nta bushyuhe bukenewe, kandi nibindi bikorwa nka protein no gukuraho RNA ntibisabwa.Umubare wa ADN yasohotse urashobora gukoreshwa nkicyitegererezo cya PCR.

2 × M-PCR Biroroshye^TMKuvanga bifite kwihanganira bikomeye PCR yerekana inhibitor, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction ya reaction, optimizer ya PCR na stabilisateur.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer NP
	Foregene Protease Yongeyeho I.
	6 × ADN Yipakurura Buffer
Igice cya II	2 × M PCR EasyTM ivanze
Amabwiriza

Ibiranga ibyiza

■Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

Icyitegererezo gikenewe ni gito, fata umurizo wimbeba (cyangwa amatwi yimbeba ifite diameter ya 2-5mm) hejuru ya 2mm.

■ Biroroshye gukora, irashobora guterwa nta gushyushya kugirango idakora protease, kandi ibicuruzwa byatewe birashobora gukorerwa PCR.

■ Foregene Imbeba Umurizo wa Genomic ADN yo gukuramo ibikoresho (DE-05211) irashobora gukoreshwa mugukuramo ADN genomic mumurizo wimbeba yatunganijwe.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.