Imbuto y'Ibihingwa by'Ubushinwa (Ntoya na Hagati) Direct PCR Kit I-UNG (idafite ibikoresho by'icyitegererezo) Ihingura n'utanga isoko |Foregene

Imbuto y'ibihingwa (Ntoya na Hagati) Direct PCR Kit I-UNG (idafite ibikoresho by'icyitegererezo)

Ibisobanuro:

Gukoresha mu buryo butaziguye imbuto lysate nk'icyitegererezo cyo kongera PCR, nta gukuramo ADN itandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gicuruzwa gikoresha uburyo bwihariye bwa lysis reaction, bushobora kurekura byihuse ADN ya genomique yintangarugero yimbuto yibihingwa hamwe na polysaccharide nkeya hamwe na polifenolike (nka: umuceri, ingano, itabi, ibigori, soya, nibindi) kugirango ikoreshwe mubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha nini nini.Kugirango uhuze ibikenewe mu bizamini bitandukanye byo gutahura, iki gikoresho kirashobora gukoresha ubwoko butandukanye bwintangarugero (nkimbuto zose, gukata uduce duto, cyangwa ingero zubutaka bwimbuto nyinshi) mubushakashatsi.Muri byo, kuburugero rwimbuto rugikeneye kumera, urashobora guhitamo guca uduce duto duto (1-5mg) usibye urusoro rwimbuto kugirango ugerageze;kubushakashatsi bugomba gukopororwa mumubare munini wintangarugero, urashobora guhitamo kuvanga ingero nyinshi hanyuma ukazihuza.Nyuma yo gusya mubice bifite diameter ya 0.5mm, ubushakashatsi burashobora gukorwa (byibuze 0.1% byurugero rwicyitegererezo birashobora kuboneka).

Ibisobanuro

50T, 200T, 500T, 2000T

Ibigize ibikoresho

Imbuto yibihingwa Direct PCR Kit I-UNG Imbuto y'ibihingwa (Ntoya na Hagati) Direct PCR Kit-UNG
Ibigize ibikoresho		TP-0312T	TP-03121	TP-03122	TP-03123
Ibigize ibikoresho		50 T.	200 T.	500 T.	2000 T.
Igice I.	Buffer SP1	10ml	40ml	100ml	400ml
	Buffer SP2	3ml	10ml	25ml	100ml
	6 × ADN Yipakurura Buffer	1.5ml	1.5ml	1.5ml	1.5ml × 4
Igice cya II	2 × Imbuto PCR byoroshye^TMKuvanga (UNG)	500μl	1ml × 2	1.7ml × 3	1.7ml × 12
Igitabo		1	1	1	1

Ibiranga ibyiza

- Ntibikenewe koza ADN bitwara igihe kandi bihenze.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Kurwanya umwanda PCR sisitemu 2 × Imbuto PCR Byoroshye^TMKuvanga (UNG) birashobora gukuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.