Amazi ADN Yigunze Igikoresho cya ADN Gukuramo no Kweza Amazi
Ibisobanuro:
Kuraho vuba ADN ya genomic yo mu rwego rwo hejuru mikorobe mvaruganda zitandukanye.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
50 Imyiteguro
-Lysozyme: mu buryo bwa hydrolyze urukuta rw'ingirabuzimafatizo ya bagiteri nziza.
-Buffer TE: ikoreshwa mugutegura 100mg / ml igisubizo cya Lysozyme no gutanga lysozyme enzymatique hydrolysis ibidukikije.
-Buffer SG1 & Buffer SG2: Tanga icyitegererezo cya protease igogora.
-Foregene Protease: Enzymatically hydrolyze sample in protease enzymatique ibidukikije kugirango irekure ADN genomic.
-Buffer SG3: Gukora Foregene Protease no gutanga ibidukikije byapakira ADN.
-Buffer SG4: Inyongera yo gutanga ibidukikije byo gupakira ADN.
-Buffer PW: Kuraho umwanda nka proteyine na RNA muri ADN.
-Buffer WB: Kuraho ion zumunyu zisigaye muri ADN.
-Buffer EB: kura ADN kumurongo wo kweza.
-DNA-Inkingi gusa: byumwihariko adsorb ADN genomic muri lysate.
Ibigize ibikoresho
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta RNase yanduye: ADN-Inkingi yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN ya genomic nta RNase yongeyeho mugihe cyibigeragezo, birinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Igikorwa kiroroshye, kandi ibikorwa byo kuvoma ADN genomic amazi birashobora kurangira muminota 40.
-Ibyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: ntagikenewe gukuramo reagent.
-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Irakwiriye kwezwa ADN ya genomic yingero zikurikira: amazi yicyuzi cyamazi yo mumazi, amazi yicyuzi, amazi ya robine, amazi yikiyaga, amazi yinzuzi, amazi yicyuzi cya lotus nizindi ngero.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C), niba gikeneye kubikwa mugihe kirekire, gishobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya muri 37°C kwiyuhagira amazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);iyo bibitswe kuri 4 ℃, ibikorwa byayo nibihinduka bizaba byiza, bityo birasabwa kubibika kuri 4 ℃, wibuke kutabika kuri -20 ℃.
-Ifu yumye Lysozyme ibikwa kuri -20 ° C;igisubizo cyateguwe na Lysozyme kigabanijwemo uduce duto kandi kibikwa kuri -20 ° C.
