Ubushinwa Tissue Yinyamanswa ADN Yigunze Igikoresho cyo gukuramo ADN Ibikoresho byo gukuramo inyamaswa zintangarugero zikora nuwabitanze |Foregene

Ibice by'inyamanswa ADN Yigunze Igikoresho cyo gukuramo ADN Ibikoresho by'inyamanswa

Ibice by'inyamanswa ADN Yigunze Igikoresho cyo gukuramo ADN Ibikoresho byo mu bwoko bw'inyamanswa z'icyitegererezo

Ibisobanuro:

Gukuramo vuba no kweza ADN genomic biva ahantu henshi, nk'inyama z'inyamaswa, selile, nibindi.

◮Umuvuduko wihuse:ibikorwa byinshi kuruta protease isa kandi igogora ingero za tissue byihuse;

◮Biroroshye:birashobora kurangira muminota 50.

◮Byoroshye:Bikorewe ku bushyuhe bwicyumba.

◮Umutekano:nta reagent ngenga ikoreshwa.

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Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gikoresho gikoresha ADN yonyine inkingi ishobora guhuza ADN, protease ya Foregene na sisitemu idasanzwe.ADN yo mu rwego rwohejuru irashobora gukurwa mu ngirabuzimafatizo zitandukanye zifite imico n’inyamanswa mu minota 30 kugeza kuri 50.

ADN yonyine ya silika gel membrane ikoreshwa muri spin inkingi ni ibikoresho bishya byihariye bya Foregene, bishobora guhuza neza na ADN, kandi bigakuraho cyane RNA, poroteyine zanduye, ion hamwe n’ibindi binyabuzima mu ngirabuzimafatizo.ADN ya 5-80μg yo mu rwego rwo hejuru irashobora kwezwa kuva 10-50mg.

Ibisobanuro

Inyamanswa yinyamanswa ADN yigunga
Ibirimo	DE-05011	DE-05012	DE-05013
Ibirimo	50T	100T	250T
Buffer L1	20ml	40ml	100ml
Buffer L2 *	20ml	40ml	100ml
Buffer PW *	25ml	50ml	125ml
Buffer WB	25ml	50ml	125ml
Buffer EB	10ml	20ml	50ml
Foregene Protease	1.25ml	2.5ml	6.5ml
ADN-Inkingi gusa	50	100	250
Igitabo	1	1	1

*: Buffer L2 na Buffer PW zirimo umunyu ushimishije.Nyamuneka kwambara uturindantoki kandi ufate ingamba zo kubarinda mugihe ukora.

Ibiranga ibyiza

■ Nta kwanduza RNase: Inkingi ya ADN yonyine yatanzwe mu gikoresho ituma bishoboka kuvana RNA muri ADN ya genomic nta RNase yongeyeho mu gihe cy’igerageza, bityo bikabuza laboratoire kwanduzwa na RNase idasanzwe.

Speed Umuvuduko wihuse: Foregene Protease ifite ibikorwa byinshi kuruta protease isa kandi igogora ingero za tissue byihuse.

Byoroshye: igikorwa cyo gukuramo ADN genomic kirashobora kurangira muminota 50.

■ Byoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, nta 4 cent ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol irakenewe.

Umutekano: nta reagent ngenga isabwa.

Quality Ubwiza buhanitse: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, birashobora kuzuza ibisabwa mubushakashatsi butandukanye.