SARS-CoV-2 Nucleic Acide Detection Kit (Multiplex PCR Fluorescent Probe Method)
Ibisobanuro:
PCR itaziguye:Ntibikenewe ko acide nucleic acide kit na Sisitemu, byujujwe ingero 96 mumasaha 1 cyangwa arenga.
Equipment Ibikoresho bike bisabwa no gukoresha byoroshye:Gusa ukeneye sisitemu nyayo-Igihe PCR.
◮ LoD hamwe no kumva neza:Kumenya gushishoza birashobora kugera kurwego rwo hasi nka 500 kopi / ml.
Gukoresha
Iki gikoresho gikoresha tekinoroji ya RT PCR (rRT-PCR) kugirango hamenyekane neza acide nucleic acide ya SARS-CoV-2 muri acide ya nasofaryngeal cyangwa oropharyngeal swab.
Ihame ryo kwipimisha
Iyi primer na probe byashizweho kugirango hamenyekane urutonde rwabitswe rwa SARS-CoV-2 (gene ya ORF1ab na N gen).Igikoresho kirimo Igenzura ryimbere rikoreshwa mugusuzuma ubuziranenge bwikigereranyo.
Ikoranabuhanga nyaryo PCR ikoresha polymerase urunigi (PCR) kugirango yongere intego yihariye
Urutonde hamwe nintego yihariye yo kumenya RNA yongerewe.Iperereza ryanditseho
umunyamakuru wa fluorescent hamwe namabara yo kuzimya.
Ibisobanuro Byihuse
◮Ibisobanuro:48 Rxns / kit, 96 Rxns / kit
Umwihariko:96,72%
Ibyiyumvo:97,92%
Ubwoko bwikigereranyo bwakoreshwa
Nasopharyngeal Swab cyangwa Oropharyngeal Swab
◮Igikoresho gikoreshwa
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Ibigize
| Oya. | Ibigize | Umubare | Ibice nyamukuru | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic aside irekura | 1.4mL / tube | Imiyoboro 2 | 5.3 mL / icupa | Icupa 1 | Surfactant |
| 2 | Kurinda RNA | 27 μL / umuyoboro | 1 tube | 53 μL / umuyoboro | 1 tube | RNase inhibitor |
| 3 | SARS-CoV-2 igisubizo cyibisubizo | 800 μL / umuyoboro | 1 tube | 1600 μL / umuyoboro | 1 tube | Primer, probe, reaction reaction, dNTP |
| 4 | SARS-CoV-2 enzyme ivanze | 80 μL / umuyoboro | 1 tube | 160 μL / umuyoboro | 1 tube | Gutangira bishyushye Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 kugenzura neza | 100 μL / umuyoboro | 1 tube | 100 μL / umuyoboro | 1 tube | Recombinant plasmid ikubiyemo igice cyagenewe, RNA |
| 6 | SARS-CoV-2 kugenzura nabi | 1200 μL / umuyoboro | 1 tube | 1200 μL / umuyoboro | 1 tube | TE buffer |
Ibiranga ibyiza
◮PCR itaziguye
Ntibikenewe ko acide nucleic acide kit na Sisitemu, byujujwe ingero 96 mumasaha 1 cyangwa arenga.
◮Ibikoresho bike bisabwa no gukoresha byoroshye
Gusa ukeneye sisitemu nyayo-Igihe PCR.
◮LoD hamwe no kumva neza
Kumenya gushishoza birashobora kugera kurwego rwo hasi nka 500 kopi / ml.
Uburyo bwo gukoresha
Uburyo 1: Uburyo bwa PCR butaziguye
Uburyo 2: Virusi RNA Yigunze + PCR
Uburyo 1: Uburyo bwa PCR butaziguye ukoreshejeIcyitegererezo cyo Kurekura
Uburyo bwo gutahura
Ubukonje bukonje Gutwara no Kubika
Ikidodo kiva mumucyo kandi kibitswe kuri -20 ± 5 ℃;
Ubuzima bwa Shelf
Umwaka 1
Gutegeka Amakuru
Video yihuse
Andika ubutumwa bwawe hano hanyuma utwohereze
