ADN ya virusi & RNA Isolation Kit ADN ya virusi na RNA yo gukuramo ibikoresho byo gutunganya ibikoresho
Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Virus RNA Nucleic Acide Gukuramo Gukuramo Isolation Kit ikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza virusi ya RNA ifite isuku nziza kandi yujuje ubuziranenge mu ngero nka plasma, serumu, amazi adafite selile, hamwe n’umuco ndengakamere.Igikoresho kongeramo byumwihariko Linear Acrylamide, ishobora gufata byoroshye RNA nkeya mubitegererezo.RNA-Inkingi yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.
Ibikoresho byose ntabwo birimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer viRW1 na Buffer viRW2 birashobora kwemeza ko aside nucleic yabonetse yabonetse idafite proteyine, nuclease cyangwa iyindi myanda, ishobora gukoreshwa mu buryo butaziguye mu bushakashatsi bw’ibinyabuzima bwa molekile.
Ibigize ibikoresho
| Acrylamide |
| Buffer DRL |
| Buffer RW1, Buffer RW2 |
| Ubusa ddH2O |
| ADN / RNA Inkingi |
| Amabwiriza |
Ibiranga ibyiza
Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Umusaruro mwinshi wa aside nucleique: ADN / RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza ADN na RNA.
Ubushobozi bunini bwo gutunganya: urugero rwa 200μl rushobora gutunganywa buri gihe.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 20.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.
Porogaramu
Irakwiriye gukuramo no kweza aside nucleic virusi mu ngero nka plasma, serumu, umubiri utagira selile hamwe numuco ndengakamere.
Urujya n'uruza rw'akazi
Igishushanyo
Ububiko hamwe nubuzima bwa Shelf
■ Iki gikoresho gishobora kubikwa amezi 24 mugihe cyumye mubushyuhe bwicyumba (15-25 ℃);niba ikeneye kubikwa igihe kirekire, irashobora kubikwa muri 2-8 ℃.
Solution Umurongo wa Acrylamide urashobora kubikwa mubushyuhe bwicyumba muminsi 7;nyuma yo kwakira ibikoresho, nyamuneka kuyikuramo no kubika kuri -20 ° C.
■ Nyuma yo kongeramo Linear Acrylamide muri Buffer DRL, irashobora kubikwa kuri 2-8 ° C kugeza kuri 48h.Nyamuneka koresha igisubizo cyiteguye.
Igitabo cyo gusesengura ibibazo
Ibikurikira nisesengura ryibibazo bishobora guhura nabyo mugukuramo virusi ya ADN / RNA, twizeye ko bizafasha mubushakashatsi bwawe.Mubyongeyeho, kubindi bibazo byubushakashatsi cyangwa tekiniki usibye amabwiriza yimikorere no gusesengura ibibazo, twatanze inkunga ya tekiniki yo kugufasha.Niba hari ibyo ukeneye, twandikire: 028-83360257 cyangwa E-imeri:
Tech@foregene.com.
Nta gukuramo aside nucleic cyangwa umusaruro wa acide nucleic
Mubisanzwe hariho ibintu byinshi bigira ingaruka kumikorere yo gukira, nka: urugero rwa acide nucleic aside, uburyo bwo gukora, ingano ya elution, nibindi.
Isesengura ry'impamvu zisanzwe:
1. Kwiyuhagira urubura cyangwa ubushyuhe buke (4 ° C) centrifugation byakozwe mugihe cyagenwe.
Igitekerezo: Kora ku bushyuhe bwicyumba (15-25 ° C) mugihe cyose, ntukarabe urubura hamwe na centrifugation yubushyuhe buke.
2. Icyitegererezo cyabitswe nabi cyangwa icyitegererezo kibitswe igihe kirekire.
Icyifuzo: Bika ingero kuri -80 ° C kandi wirinde gukonjesha no gukonja inshuro nyinshi;gerageza gukoresha ingero zegeranijwe kugirango zivemo nucleic aside.
3. Lisisi ntangarugero idahagije.
Icyifuzo: Nyamuneka menya neza ko sample na lysis igisubizo cyakazi kivanze neza kandi kigashyirwa mubushyuhe bwicyumba (15-25 ° C) muminota 10.
4. Kwiyongera nabi kwa eluent.
Icyifuzo: Menya neza ko RNase-yubusa ddH2O yongewemo ibitonyanga hagati yinkingi yo kweza inkingi, kandi ntukayijugunye kumpeta yo kweza.
5. Ingano yukuri ya Ethanol yuzuye ntabwo yongerewe kuri Buffer RW2.
Igitekerezo: Nyamuneka kurikiza amabwiriza, ongeramo ingano yukuri ya Ethanol yuzuye kuri Buffer RW2 hanyuma uvange neza mbere yo gukoresha ibikoresho.
6. Ingano yicyitegererezo idakwiye.
Igitekerezo: 200µl yicyitegererezo gitunganyirizwa kuri 500µl ya Buffer DRL.Gutunganya urugero rwinshi bizavamo umusaruro muke wa nucleic aside.
7. Ingano ya elution idakwiye cyangwa gusiba kutuzuye.
Icyifuzo: Ingano nziza yinkingi yo kweza ni 30-50μl;niba ingaruka zo gukuraho zidashimishije, birasabwa kongera igihe mubushyuhe bwicyumba nyuma yo kongeramo ubushyuhe bwa RNase-Free ddH2O, nka 5-10min.
8. Ethanol iguma ku nkingi nyuma yo gukaraba hamwe na Buffer RW2.
Igitekerezo: Niba Ethanol igumye nyuma ya centrifugation hamwe na Buffer RW2 muminota 2, inkingi irashobora gushyirwa mubushyuhe bwicyumba muminota 5 nyuma ya centrifugation kugirango ikureho Ethanol isigaye.
Acide nucleic isukuye yangiritse
Ubwiza bwa acide nucleic isukuye ifitanye isano no kubungabunga icyitegererezo, kwanduza RNase, imikorere nibindi bintu.Isesengura ry'impamvu zisanzwe:
1. Ingero zegeranijwe ntizibitswe mugihe.
Igitekerezo: Niba icyitegererezo kidakoreshejwe mugihe cyo gukusanya, nyamuneka ubike kuri -80 ° C mubushyuhe buke ako kanya.Kubikuramo RNA, gerageza gukoresha ingero zegeranijwe.
2. Kusanya ingero hanyuma uhagarike kandi ushushe inshuro nyinshi.
Igitekerezo: Irinde gukonjesha no gukonjesha (bitarenze inshuro imwe) mugihe cyo gukusanya no kubika ingero, bitabaye ibyo umusaruro wa aside nucleique uzagabanuka.
3. RNase itangirwa mucyumba cyo gukoreramo cyangwa uturindantoki twajugunywe, masike, nibindi ntabwo byambarwa.
Icyifuzo: Ubushakashatsi bwo gukuramo RNA bukorwa neza mubyumba bitandukanye bya RNA, kandi ameza ya laboratoire agomba gusukurwa mbere yubushakashatsi.
Wambare uturindantoki hamwe na masike mugihe cyo kugerageza kugirango wirinde kwangirika kwa RNA guterwa no kwinjiza RNase kurwego runini.
4. Reagent yandujwe na RNase mugihe cyo kuyikoresha.
Icyifuzo: Simbuza ADN nshya ya virusi ya RNA / RNA yo kwigunga kubushakashatsi bujyanye nayo.
5. Imiyoboro ya centrifuge hamwe na pipette ikoreshwa muri RNA manipulation yanduye na RNase.
Igitekerezo: Menya neza ko imiyoboro ya centrifuge, inama za pipeti, imiyoboro, nibindi bikoreshwa mugukuramo RNA byose ni RNase-Free.
Acide nucleic isukuye igira ingaruka kubushakashatsi bwo hasi
ADN na RNA byahanaguwe ninkingi yo kweza, niba umunyu ion hamwe na proteyine biri hejuru cyane, bizagira ingaruka kubigeragezo byo hepfo, nka: Kwiyongera kwa PCR, kwandukura, nibindi.
1. ADN yatowe na RNA ifite ion zumunyu zisigaye.
Igitekerezo: Menya neza ko ingano yukuri ya Ethanol yuzuye yongewe kuri Buffer RW2, hanyuma ukarabe inkingi yo kweza kabiri kumuvuduko wa centrifugation yerekanwe mumabwiriza yo gukora;Kora centrifugation kugirango ugabanye umunyu ion.
2. ADN yatowe na RNA bifite ibisigisigi bya Ethanol.
Igitekerezo: Nyuma yo kwemeza koza hamwe na Buffer RW2, kora centrifugation yubusa kuri centrifugation yihuta mumabwiriza yo gukora;niba hakiri ibisigisigi bya Ethanol, urashobora centrifuge umuyoboro wubusa hanyuma ukabishyira mubushyuhe bwicyumba muminota 5 kugirango ukureho ibisigazwa bya Ethanol kurwego runini.
Imfashanyigisho:
Virusi ya ADN & RNA Igitabo cyo Kwigunga
