Bagiteri ya ADN Yigunze Kit Bakteri ya Genomic Gukuramo ibikoresho byo kweza
Ibisobanuro:
Byihuse usukure ADN ya genomic yo mu rwego rwo hejuru muri bagiteri mugice cyo gukura kwa logarithmic.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Iki gikoresho gitanga uburyo bwihuse kandi bworoshye bwo gukuramo ADN genomic mumico ya bagiteri (Gram-negative na Gram-positif) ahantu hatandukanye;irashobora gutunganya munsi ya 3ml yimico ya bagiteri mugice cyo gukura kwa logarithmic (bagiteri 1 × 109).Igikoresho cyahujwe na Protease ikora neza cyane, ituma 15-50μg ya ADN yo mu rwego rwo hejuru ikuramo ADN mu isaha imwe.Mubyongeyeho, ibikoresho birashobora kandi gukuramo ibikoresho bya geneti bitari genome, nka plasmide, Cosmid, BAC, nibindi.
Ibikoresho bya ADN byonyine bya silika gel matrix bikoreshwa mukuzunguruka ni ibintu bishya bidasanzwe byikigo, bishobora gukora neza kandi byumwihariko kwamamaza ADN.Ubushobozi ntarengwa bwa adsorption kuri ADN ni 80μg.Sisitemu idasanzwe na sisitemu yo gukuraho irashobora gukuraho cyane RNA, poroteyine zanduye, ion hamwe n’ibindi binyabuzima mu ngirabuzimafatizo.Igice cya ADN cyakuweho ni kinini, cyera cyane, gihamye kandi cyizewe, kandi ubunini bwa ADN buhagaze neza kuri 23kb.
Ibisobanuro
50 Imyiteguro, 100 Itegura, 250 Itegura
Ibigize ibikoresho
| Buffer ML1 |
| Buffer ML2 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| Lysozyme |
| Buffer TE |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Ntibikenewe ko wongera RNase kugirango ukureho RNA muri ADN genomic, wirinde laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease ifite ibikorwa byinshi kurenza protease isa kandi irashobora gusya ingero za bagiteri zacitse mugihe gito.
-Ibyoroshye: Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Iki gikoresho gikwiranye no kweza ADN ya genomique yintangarugero zikurikira: Bagiteri ya Gram-mbi na bagiteri nziza ya Gram mugice cyo gukura kwa logarithmic.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);iyo bibitswe kuri 4 ° C, ibikorwa byayo no gutuza bizaba byiza, bityo birasabwa kubibika kuri 4 ° C, ibuka kutabika kuri -20 ° C.
-Lysozyme ni ifu yumye, ikora iyo ibitswe kuri 2-8 ° C mugihe kirekire (amezi 3).Kubikwa igihe kirekire, nyamuneka ubike kuri -20 ° C.
