RT-PCR Byoroshyeᵀᴹ II (Intambwe ebyiri)
Ibisobanuro:
◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
◮ Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
Ibisobanuro
RT-PCR BiroroshyeTM II(Intambwe ebyiri) ikubiyemo reagent zose zintambwe ebyiri RT-PCR, ihuza reaction ya RT hamwe na PCR reaction mugikoresho kimwe, itanga RT ivanze neza na PCR ivanze.Imvange idasanzwe ya RT irashobora guhuza ubuziranenge-bwohejuru bwa mbere cDNA byoroshye kandi neza. 2× PCRBiroroshyeTM KuvangaByongeye ifite imikorere-yo hejuru kandi yongerewe imbaraga.Ihuriro ryibinyabuzima byombi bituma kumenya gene igenewe kurushaho.
Ibigize ibikoresho
| RT-PCRBiroroshyeTM II (Intambwe ebyiri) | ||
| Ibirimo | RT-02021 | RT-02022 |
| 100T(20μlSisitemu) | 500T(20μlSisitemu) | |
| 2 × RT BiroroshyeTM Kuvanga | 1ml | 1.7ml ×3 |
| 2× PCRBiroroshyeTM KuvangaByongeye | 1ml | 1.7ml ×3 |
| Primer Primer (50)μM) | 200μl | 1ml |
| Oligo (dT) 18 Primer (50μM) | 200μl | 1ml |
| Ubusa ddH2O | 1.7ml | 1.7ml |
| Igitabo gikubiyemo amabwiriza | 1igice | 1igice |
Ibiranga ibyiza
-Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
-Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
-Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
1.Imiterere yo gutwara abantu
Inzira yose yubushyuhe bwo hasi yubushyuhe bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ° C.
2. Ububiko
Igikoresho kibitswe kuri -20 ° C.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20 ° C ako kanya ukimara kuyakira.Niba uburyo bwo kubika bukwiye,tibicuruzwa ntabwo bizatesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
