TP53 / CSP17 Amabara abiri
Ibisobanuro:
Dukurikije ihame rya ADN yuzuzanya, iperereza rya TP53 orange-umutuku na CSP17 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Dukurikije ihame rya ADN yuzuzanya, iperereza rya TP53 orange-umutuku na CSP17 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| Ibizamini 5 | Ibizamini 10 | Ibizamini 20 | |
| TP53 / CSP17 Amabara abiri | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane ubuziranenge bwa gene ya TP53 yumuntu mubitegererezo bya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
- Icyitegererezo
Ingero za paraffin zashyizwemo ingero zashyizwe muri 10% itagira aho ibogamiye, kandi uburebure bwibice bwari hagati ya 3 na 5 µm.
- Ingero z'akagari
Kubijyanye n'amagufwa cyangwa amaraso ya peripheri, icyitegererezo gishya kidakwiye kubikwa kuri 4 ° C mugihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo bigomba kubikwa kuri -20 ° C.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
