BCL6 Amabara abiri Kumena Uretse Probe
Ibisobanuro:
Dukurikije ihame ryo kuzuzanya gushingiye kuri ADN, iperereza rya BCL6 orange na BCL6 icyatsi kibisi ryakoreshejwe kugirango rihuze hamwe na ADN ikurikiranye muri nucleus, kandi amakuru yimiterere ya gene muri nucleus yagaragaye kandi asesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Dukurikije ihame ryo kuzuzanya gushingiye kuri ADN, iperereza rya BCL6 orange na BCL6 icyatsi kibisi ryakoreshejwe kugirango rihuze hamwe na ADN ikurikiranye muri nucleus, kandi amakuru yimiterere ya gene muri nucleus yagaragaye kandi asesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| Ibizamini 5 | Ibizamini 10 | Ibizamini 20 | |
| BCL6 Amabara abiri Kumena Uretse Probe | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane neza imiterere idasanzwe ya gene ya BCL6 yabantu mu cyitegererezo cya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
-Tissue ingero
Ingero za paraffin zashyizwemo ingero zashyizwe muri 10% itagira aho ibogamiye, kandi uburebure bwibice bwari hagati ya 3 na 5 µm.
-Akagari ingero
Kubijyanye n'amagufwa cyangwa amaraso ya peripheri, icyitegererezo gishya kitarashyizweho cyabitswe kuri 4 ℃ mu gihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo byabitswe kuri 20 ℃.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
